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Literature summary extracted from

  • Sung, P.-H.; Huang, F.-C.; Do, Y.-Y.; Huang, P.-L.
    Functional expression of geraniol 10-hydroxylase reveals its dual function in the biosynthesis of terpenoid and phenylpropanoid (2011), J. Agric. Food Chem., 59, 4637-4643.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 co-expression of G10H with the NADPH-cytochrome P450 reductase from Arabidopsis thaliana in Spodoptera frugiperda Sf9 cell microsomes using the baculovirus transfection method, quantitative expression analysis Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.83 0.0158
-
 geraniol pH 8.0, 30°C, recombinant enzyme Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.83 membrane
-
 Catharanthus roseus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus Q8VWZ7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.83 leaf
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 additional information CrG10H also catalyzed 3'-hydroxylation of the flavanone naringenin to form eriodictyol, a reaction of the flavonoid 3'-hydroxylase. Analysis of the enzyme reactions of the recombinant enzyme with naringenin, apigenin, kaempferol, pcoumaric acid, and ferulic acid, mass spectrometric product analysis, overview Catharanthus roseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 G10H
-
 Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.83 30
-
 assay at Catharanthus roseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.83 0.131
-
 geraniol pH 8.0, 30°C, recombinant enzyme Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.83 8
-
 assay at Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.14.14.83 metabolism G10H catalyzes the first committed step of the biosynthetic pathway of strictosidine and other terpenoid indole alkaloids, overview Catharanthus roseus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.83 0.000008
-
 geraniol pH 8.0, 30°C, recombinant enzyme Catharanthus roseus