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Literature summary extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.
    Vanadate inhibition of fungal PhyA and bacterial AppA2 histidine acid phosphatases (2011), J. Agric. Food Chem., 59, 1739-1743.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 metavanadate 50% inhibition at 0.0011 and pH 2.5, or at 0.0053 mM and pH 5.0 Aspergillus niger
3.1.3.8 metavanadate 50% inhibition at 0.00075 mM and pH 5.0 or at 0.00090 mM and pH 3.0 Escherichia coli
3.1.3.8 orthovanadate 50% inhibition at 0.0004 and pH 2.5, or at 0.040 mM and pH 5.0 Aspergillus niger
3.1.3.8 orthovanadate 50% inhibition at 0.0022 mM and pH 5.0 or at 0.000072 mM and pH 3.0 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.087 0.324 phytate pH 2.5, 55°C Aspergillus niger
3.1.3.8 0.25 0.425 phytate pH 4.0, 55°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 phytate + H2O Aspergillus niger
-
 ? + phosphate
-
 ?
3.1.3.8 phytate + H2O Escherichia coli
-
 ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
 gene phyA
-
3.1.3.8 Escherichia coli Q6RK08 gene AppA2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 phytate + H2O
-
 Aspergillus niger ? + phosphate
-
 ?
3.1.3.8 phytate + H2O
-
 Escherichia coli ? + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 AppA2
-
 Escherichia coli
3.1.3.8 histidine acid phosphatase
-
 Aspergillus niger
3.1.3.8 histidine acid phosphatase
-
 Escherichia coli
3.1.3.8 PhyA
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
 assay at Aspergillus niger
3.1.3.8 55
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 1260
-
 phytate pH 2.5, 55°C Aspergillus niger
3.1.3.8 2348 4470 phytate pH 4.0, 55°C Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2.5 5
-
 Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.8 additional information
-
 additional information vanadate inhibition kinetics Aspergillus niger
3.1.3.8 additional information
-
 additional information vanadate inhibition kinetics Escherichia coli
3.1.3.8 0.0002
-
 orthovanadate pH 4.0, 55°C, 0.0005 mM orthovanadate Escherichia coli
3.1.3.8 0.004
-
 metavanadate pH 4.0, 55°C, 0.010 mM metavanadate Escherichia coli
3.1.3.8 0.0056
-
 metavanadate pH 2.5, 55°C, 0.015 mM metavanadate Aspergillus niger
3.1.3.8 0.0057
-
 orthovanadate pH 2.5, 55°C, 0.010 mM orthovanadate Aspergillus niger

General Information

EC Number General Information Comment Organism
3.1.3.8 additional information the enzyme can also act as a vanadate haloperoxidase, EC 1.11.1.10 Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 0.0039
-
 phytate pH 2.5, 55°C Aspergillus niger
3.1.3.8 0.0093 0.01 phytate pH 4.0, 55°C Escherichia coli