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Literature summary extracted from

  • Savary, B.; Vasu, P.; Nunez, A.; Cameron, R.
    Identification of thermolabile pectin methylesterases from sweet orange fruit by peptide mass fingerprinting (2010), J. Agric. Food Chem., 58, 12462-12468.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 34341
-
 x * 34341, isoform PME4, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 34467
-
 x * 34467, isoform PME2, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 34485
-
 x * 34485, isoform PME1, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 35000
-
 x * 35000, isoform PME2, SDS-PAGE Citrus sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Citrus sinensis
-
-
-
3.1.1.11 Citrus sinensis P83948 isoform PME3
-
3.1.1.11 Citrus sinensis Q8GS16 isoform PME4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 affinity purification with immobilized pectin methylesterase-inhibitor protein Citrus sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.11 fruit
-
 Citrus sinensis
-
3.1.1.11 fruit isoform PME1 is a minor citrus fruit thermolabile pectin methylesterase Citrus sinensis
-
3.1.1.11 fruit isoform PME2 is the major thermolabile pectin methylesterase isoenzyme accumulated in citrus pulp tissue Citrus sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Citrus sinensis methanol + pectate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 34341, isoform PME4, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 ? x * 34467, isoform PME2, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 ? x * 34485, isoform PME1, MALDI-TOF mass spectrometry Citrus sinensis
3.1.1.11 ? x * 35000, isoform PME2, SDS-PAGE Citrus sinensis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 PME1 isoform Citrus sinensis
3.1.1.11 PME2 isoform Citrus sinensis
3.1.1.11 PME3 isoform Citrus sinensis
3.1.1.11 PME4 isoform Citrus sinensis
3.1.1.11 thermolabile pectin methylesterase
-
 Citrus sinensis
3.1.1.11 TL-PME
-
 Citrus sinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 70
-
 following heating of a crude pulp tissue cell wall extract at 70°C, the activity for the purified isoform PME2 is rapidly lost Citrus sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 5.5 6 in the presence of 1.2% NaCl Citrus sinensis