BRENDA - Enzyme Database

Analysis of human ferrochelatase iron binding via amide hydrogen/deuterium exchange mass spectrometry

Asuru, A.; Busenlehner, L.; Int. J. Mass Spectrom. 302, 76-84 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.99.1.1
expressed in Escherichia coli HB101 cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.99.1.1
mitochondrion
inner mitochondrial membrane
Homo sapiens
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.99.1.1
protoporphyrin IX + Fe2+
Homo sapiens
-
protoheme IX + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.99.1.1
Homo sapiens
P22830
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.99.1.1
Ni-NTA column chromatography. After aerobic purification, the cysteine residues can oxidize and release the [2Fe-2S] cluster, causing ferrochelatase to precipitate. CHAPS is best at solubilizing ferrochelatase, while retaining full activity as compared to the enzyme in sodium cholate
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.99.1.1
mesoporphyrin IX + Fe2+
-
715203
Homo sapiens
protoporphyrin IX + H+
-
-
-
?
4.99.1.1
protoporphyrin IX + Fe2+
-
715203
Homo sapiens
protoheme IX + H+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.99.1.1
[2Fe-2S]-center
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.99.1.1
expressed in Escherichia coli HB101 cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.99.1.1
[2Fe-2S]-center
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.99.1.1
mitochondrion
inner mitochondrial membrane
Homo sapiens
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.99.1.1
protoporphyrin IX + Fe2+
Homo sapiens
-
protoheme IX + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.99.1.1
Ni-NTA column chromatography. After aerobic purification, the cysteine residues can oxidize and release the [2Fe-2S] cluster, causing ferrochelatase to precipitate. CHAPS is best at solubilizing ferrochelatase, while retaining full activity as compared to the enzyme in sodium cholate
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.99.1.1
mesoporphyrin IX + Fe2+
-
715203
Homo sapiens
protoporphyrin IX + H+
-
-
-
?
4.99.1.1
protoporphyrin IX + Fe2+
-
715203
Homo sapiens
protoheme IX + H+
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
4.99.1.1
metabolism
ferrochelatase catalyzes the last step in the heme biosynthetic pathway
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.99.1.1
metabolism
ferrochelatase catalyzes the last step in the heme biosynthetic pathway
Homo sapiens