Literature summary extracted from

Kaur, N.; Gautam, A.; Kumar, S.; Singh, A.; Singh, N.; Sharma, S.; Sharma, R.; Tewari, R.; Singh, T.P.
Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa (2011), Int. J. Biol. Macromol., 48, 779-787.

Application

EC Number	Application	Comment	Organism
4.3.3.7	drug development	the intracellular enzyme dihydrodipicolinate synthase a potential drug target because it is essential for the growth of bacteria while it is absent in humans	Pseudomonas aeruginosa

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.3.3.7	gene dapA, DNA and amino acid sequence determination and analysis, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.3.3.7	purified recombinant DHDPS, free or in complex with inhibitor (S)-lysine, 15 mg/ml protein in 50 mM Tris-HCl, pH 8.5, at 20°C using hanging drop vapour diffusion method, mixing of 0.005 ml protein solution with 0.005 ml well solution containing 30% w/v PEG-3350, 170 mM MgCl2, 70 mM Tris-HCl, pH 8.5, and 6% v/v propylene glycol. Crystals obtained without 6% propylene glycol are soaked in the reservoir containing 20 mg/ml (S)-lysine, X-ray diffraction structure determination and analysis at 2.65-2.85 A resolution	Pseudomonas aeruginosa

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.3.3.7	L-lysine	binds at three sites to the enzyme, binding structure, overview	Pseudomonas aeruginosa

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.3.3.7	0.17	-	L-aspartate 4-semialdehyde	pH 8.0, 37°C, recombinant His-tagged enzyme	Pseudomonas aeruginosa
4.3.3.7	0.9	-	pyruvate	pH 8.0, 37°C, recombinant His-tagged enzyme	Pseudomonas aeruginosa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
4.3.3.7	intracellular	-	Pseudomonas aeruginosa	5622	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.3.3.7	L-aspartate 4-semialdehyde + pyruvate	Pseudomonas aeruginosa	-	(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.3.3.7	no activity in Homo sapiens	-	-	-
4.3.3.7	Pseudomonas aeruginosa	Q9I4W3	gene dapA	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.3.3.7	recombinant His-tagged DHDPS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.3.3.7	L-aspartate 4-semialdehyde + pyruvate	-	Pseudomonas aeruginosa	(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O	-	?
4.3.3.7	additional information	the active site is formed by residues Thr44, Tyr107 and Tyr133, stereochemically suitable for catalytic function	Pseudomonas aeruginosa	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.3.3.7	dimer	three-dimensional structure determination shows that DHDPS forms a homodimer which is stabilized by several hydrogen bonds and van der Waals forces at the interface, active site structure, overview. Each monomer is composed of two domains, the N-terminal domain consists of residues 1-224, and forms an 8-fold parallel alpha/beta barrel	Pseudomonas aeruginosa
4.3.3.7	More	the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
4.3.3.7	DHDPS	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4.3.3.7	37	-	-	Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4.3.3.7	65	-	the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C	Pseudomonas aeruginosa

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.3.3.7	8	-	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)