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Literature summary extracted from

  • Lumjuan, N.; Rajatileka, S.; Changsom, D.; Wicheer, J.; Leelapat, P.; Prapanthadara, L.A.; Somboon, P.; Lycett, G.; Ranson, H.
    The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides (2011), Insect Biochem. Mol. Biol., 41, 203-209.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 the Epsilon GST class in Aedes aegypti consists of eight sequentially arranged genes spanning 53645 bp on super contig 1.291, which maps to chromosome 2, quantitative RT-PCR isozyme GSTE1-GSTE8 expression analysis in different strains, overview. Overexpression of N-terminally His6-tagged Epsilon GST isozymmes GSTe1, GSTe2, GSTe8, GSTe5, and GSTe7, in Escherichia coli strain BL21 Star (DE3) Aedes aegypti

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.5.1.1 permethrin uncompetitive inhibitor of isoform GSTE2-2 Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.03
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.05
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.08
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.19
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.35
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.41
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.46
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 2.2
-
glutathione recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 3
-
glutathione recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 3.8
-
glutathione recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 4.1
-
glutathione recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 7
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 13.2
-
glutathione recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 13.6
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 26.1
-
glutathione recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 113
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.18 additional information Aedes aegypti only isozymes GSTE2-2 and GSTE5-5 are able to metabolize DDT to DDE ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Aedes aegypti
-
PMD and PMD-R strains, the latter is a highly resistant strain, Isla Mujeres (IM) strain, New Orleans (NO) susceptible strain, isozymes GSTE1-GSTE8
-
4.5.1.1 Aedes aegypti
-
strains PMD-R
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant N-terminally His6-tagged Epsilon GSTs from Escherichia cli by nickel affinity chromatography and gel filtration Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 larva
-
Aedes aegypti
-
2.5.1.18 additional information developmental stage- and gender-specific expression of isozymes GSTE1-GSTE8, overview Aedes aegypti
-
2.5.1.18 pupa
-
Aedes aegypti
-
4.5.1.1 adult
-
Aedes aegypti
-
4.5.1.1 larva
-
Aedes aegypti
-
4.5.1.1 pupa
-
Aedes aegypti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 additional information
-
of the seven Epsilon GSTs GSTE1-GSTE5, GSTE7 and GSTE8, GSTE3-3 has the highest maximum velocity of 61.5 micromol/min/mg protein with highest turnover rates being achieved by GSTE3-3 and GSTE5-5 Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
Aedes aegypti S-(2,4-dinitrophenyl)glutathione + HCl
-
?
2.5.1.18 additional information only isozymes GSTE2-2 and GSTE5-5 are able to metabolize DDT to DDE Aedes aegypti ?
-
?
4.5.1.1 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane the reaction requires the presence of GSH Aedes aegypti 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 Epsilon glutathione transferase
-
Aedes aegypti
2.5.1.18 Epsilon GST
-
Aedes aegypti
2.5.1.18 GSTE1
-
Aedes aegypti
2.5.1.18 GSTE2
-
Aedes aegypti
2.5.1.18 GSTE3
-
Aedes aegypti
2.5.1.18 GSTE4
-
Aedes aegypti
2.5.1.18 GSTE5
-
Aedes aegypti
2.5.1.18 GSTE6
-
Aedes aegypti
2.5.1.18 GSTE7
-
Aedes aegypti
2.5.1.18 GSTE8
-
Aedes aegypti
4.5.1.1 DDT dehydrochlorinase
-
Aedes aegypti
4.5.1.1 GSTE2-2 isoform Aedes aegypti
4.5.1.1 GSTE5-5 isoform Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 6
-
glutathione recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 6
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 7
-
glutathione recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 7
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 10
-
glutathione recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 10
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 11
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 11
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 23
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 23
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 23
-
glutathione recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 23
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 46
-
glutathione recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 46
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 51
-
glutathione recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 51
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
4.5.1.1 glutathione
-
Aedes aegypti

General Information

EC Number General Information Comment Organism
2.5.1.18 malfunction partial silencing of either GSTe7 or GSTe2 by RNA interference results in an increased susceptibility to the pyrethroid, deltamethrin Aedes aegypti
2.5.1.18 physiological function GSTE2 is implicated in conferring resistance to DDT for the dengue vector Aedes aegypti. Epsilon GSTs have DDT dehydrochlorinase activity, GSTe2 and two additional Epsilon GST genes, GSTe5 and GSTe7, are expressed at elevated levels in the resistant population and the recombinant homodimer GSTE5-5 also exhibits low levels of DDT dehydrochlorinase activity Aedes aegypti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.4
-
glutathione recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 0.8
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 1.4
-
glutathione recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 3.2
-
glutathione recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 3.3
-
glutathione recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 3.9
-
glutathione recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 5.9
-
glutathione recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 9
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 15
-
glutathione recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 15
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 17
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 111
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 220
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 268
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 282
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication Aedes aegypti
2.5.1.18 763
-
1-chloro-2,4-dinitrobenzene recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication Aedes aegypti