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Literature summary extracted from

  • Ilg, T.; Schmalz, S.; Werr, M.; Cramer, J.
    Acetylcholinesterases of the cat flea Ctenocephalides felis: identification of two distinct genes and biochemical characterization of recombinant and in vivo enzyme activities (2010), Insect Biochem. Mol. Biol., 40, 153-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 expressed in Pichia pastoris and in HEK-293 cells Ctenocephalides felis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 Carbaryl i.e. N-methyl-1-naphthylcarbamate, complete inhibition at concentrations exceeding 100 nM; i.e. N-methyl-1-naphthylcarbamate, complete inhibition at concentrations exceeding 100 nM Ctenocephalides felis
3.1.1.7 galanthamine
-
 Ctenocephalides felis
3.1.1.7 propoxur i.e. 2-isopropoxyphenyl-N-methylcarbamate, complete inhibition at concentrations exceeding 100 nM; i.e. 2-isopropoxyphenyl-N-methylcarbamate, complete inhibition at concentrations exceeding 100 nM Ctenocephalides felis
3.1.1.7 tacrine
-
 Ctenocephalides felis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.0158
-
 propionylthiocholine isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis
3.1.1.7 0.0243
-
 acetylthiocholine isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis
3.1.1.7 0.0244
-
 butyrylthiocholine isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis
3.1.1.7 0.0376
-
 propionylthiocholine isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis
3.1.1.7 0.0484
-
 acetylthiocholine isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis
3.1.1.7 0.0684
-
 butyrylthiocholine isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.7 cell surface isoform AChE1 possesses glycosylphosphatidylinositol membrane anchors and are transported to the cell surface Ctenocephalides felis 9986
-
3.1.1.7 cell surface isoform AChE2 possesses glycosylphosphatidylinositol membrane anchors and is transported to the cell surface Ctenocephalides felis 9986
-
3.1.1.7 membrane the majority (81%) of the AChE activity in fleas is membrane-bound Ctenocephalides felis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Ctenocephalides felis D2U6X5 cat flea
-
3.1.1.7 Ctenocephalides felis D2U6X6 cat flea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 Ni2+-NTA agarose column chromatography Ctenocephalides felis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine + H2O
-
 Ctenocephalides felis thiocholine + acetate
-
 ?
3.1.1.7 butyrylthiocholine + H2O
-
 Ctenocephalides felis thiocholine + butyrate
-
 ?
3.1.1.7 additional information isoform AChE1 is the dominant activity in Ctenocephalides felis imagoes Ctenocephalides felis ?
-
 ?
3.1.1.7 propionylthiocholine + H2O
-
 Ctenocephalides felis thiocholine + propionate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE1 isoform Ctenocephalides felis
3.1.1.7 AChE2 isoform Ctenocephalides felis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.000009
-
 isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis Carbaryl
3.1.1.7 0.000012
-
 isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis propoxur
3.1.1.7 0.000024
-
 isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis propoxur
3.1.1.7 0.000038
-
 isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis Carbaryl
3.1.1.7 0.000126
-
 isoform AChE1, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis tacrine
3.1.1.7 0.00208
-
 isoform AChE2, in 10 mM Na2HPO4/NaH2PO4, pH 7.4, pH, at 22°C Ctenocephalides felis tacrine