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Literature summary extracted from

  • Basu, M.; Weng, S.A.; Tang, H.; Khan, F.; Rossi, F.; Basu, S.
    Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma: purification and kinetic studies; synthesis of neolacto and polylactosamine core (1996), Glycoconj. J., 13, 423-432.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.275 purified enzyme is partially stabilized in the presence of phospholipid liposomes Mus musculus
2.4.1.275 the purified GalT-4 is very unstable and loses its enzymatic activity within 48 h. The enzymatic activity could be partially stabilized in the presence of N-acetylglucosamine and Mn2+, at least for 2 weeks at 4°C Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.275 EDTA completely inhibited by addition of EDTA Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.275 0.11
-
 N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide pH 7.2, 37°C Mus musculus
2.4.1.275 0.25
-
 UDP-galactose pH 7.2, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.275 membrane
-
 Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.275 Ca2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
2.4.1.275 Fe3+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
2.4.1.275 Mg2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
2.4.1.275 Mn2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.275 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.275
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.275 P-1798 cell mineral oil induced Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.275 1.344
-
 pH 7.2, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.275 UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide
-
 Mus musculus ?
-
 ?
2.4.1.275 UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide absolute requirement for an N-acetylglucosamine residue in the substrate since the lyso-derivative of the acceptor glycolipid is completely inactive as substrate Mus musculus UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.275 GalT-4
-
 Mus musculus
2.4.1.275 UDP-Gal:GLcNAcbeta1-3Galbeta1-4Glc-Cer beta1-4galactosyltransferase
-
 Mus musculus
2.4.1.275 UDP-Gal:Lc3 beta1-4galactosyltransferase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.275 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.275 6.5 7
-
 Mus musculus