EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.165 | expressed in Escherichia coli BL21(DE3) cells | Photobacterium profundum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 0.69 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 1.2 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 1.4 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 2.5 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 4.5 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 4.7 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 17.5 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 18.8 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 28.6 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.165 | Photobacterium profundum | - |
- |
- |
3.2.1.165 | Photobacterium profundum SS9 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.165 | Ni-NTA agarose column chromatography | Photobacterium profundum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 0.005 | - |
crude extract, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C | Photobacterium profundum |
3.2.1.165 | 0.046 | - |
purified enzyme, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C | Photobacterium profundum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.165 | 4-nitrophenyl beta-D-glucosaminide + H2O | - |
Photobacterium profundum | 4-nitrophenol + beta-D-glucosamine | - |
? | |
3.2.1.165 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Photobacterium profundum | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.165 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Photobacterium profundum SS9 | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.165 | cellobiose + H2O | - |
Photobacterium profundum | ? | - |
? | |
3.2.1.165 | cellobiose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
3.2.1.165 | cellopentaose + H2O | - |
Photobacterium profundum | ? | - |
? | |
3.2.1.165 | cellopentaose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
3.2.1.165 | cellotetraose + H2O | - |
Photobacterium profundum | ? | - |
? | |
3.2.1.165 | cellotriose + H2O | - |
Photobacterium profundum | ? | - |
? | |
3.2.1.165 | cellotriose + H2O | - |
Photobacterium profundum SS9 | ? | - |
? | |
3.2.1.165 | chitobiose + H2O | - |
Photobacterium profundum | D-glucosamine | - |
? | |
3.2.1.165 | deacetylated chitosan + H2O | slight hydrolysis | Photobacterium profundum | ? | - |
? | |
3.2.1.165 | GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
3.2.1.165 | GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
3.2.1.165 | GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O | - |
Photobacterium profundum | GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine | - |
? | |
3.2.1.165 | additional information | the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose | Photobacterium profundum | ? | - |
? | |
3.2.1.165 | additional information | the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose | Photobacterium profundum SS9 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.165 | exo-beta-D-glucosaminidase | - |
Photobacterium profundum |
3.2.1.165 | PBPRA0520 protein | - |
Photobacterium profundum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 50 | - |
in 50 mM phosphate buffer at pH 7.0 | Photobacterium profundum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 0.087 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 0.19 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 47.7 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 52 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 54.2 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 68.8 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 77.4 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 97.6 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 152.9 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 7 | - |
at 37°C | Photobacterium profundum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 6 | 9 | - |
Photobacterium profundum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 0.043 | - |
4-nitrophenyl beta-D-glucoside | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 0.126 | - |
4-nitrophenyl beta-D-glucosaminide | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 2.4 | - |
cellobiose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 2.5 | - |
cellopentaose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 2.7 | - |
cellotriose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 2.9 | - |
cellotetraose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 31.3 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 32.6 | - |
chitobiose | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 41.9 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum | |
3.2.1.165 | 69.2 | - |
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN | pH 7.0, 37°C | Photobacterium profundum |