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Literature summary extracted from

  • Mitra, S.; Cui, J.; Robbins, P.W.; Samuelson, J.
    A deeply divergent phosphoglucomutase (PGM) of Giardia lamblia has both PGM and phosphomannomutase activities (2010), Glycobiology, 20, 1233-1240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed in Eascherichia coli and Saccharomyces cerevisiae BY4741 wild type and pgm1DELTA/pgm2DELTA mutant strain Giardia intestinalis
5.4.2.8 expressed in Saccharomyces cerevisiae strain BY4741 Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.003
-
alpha-D-glucose 1-phosphate PGM2, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
5.4.2.2 0.0082
-
alpha-D-glucose 1-phosphate PGM1, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
5.4.2.8 0.0056
-
alpha-D-mannose 1-phosphate in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Giardia intestinalis
-
-
-
5.4.2.8 Giardia intestinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 Ni-Sepharose affinity column chromatography Giardia intestinalis
5.4.2.8 Ni-Sepharose column chromatography Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 76
-
PGM2, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
5.4.2.2 87
-
PGM1, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
5.4.2.8 25
-
using alpha-D-mannose 1-phosphate as substrate, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
Giardia intestinalis alpha-D-glucose 6-phosphate
-
?
5.4.2.8 alpha-D-mannose 1-phosphate
-
Giardia intestinalis alpha-D-mannose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PGM1 PGM1 has phosphoglucomutase activity only Giardia intestinalis
5.4.2.2 PGM2 the enzyme from Giardia lamblia has both phosphoglucomutase and phosphomannomutase activities Giardia intestinalis
5.4.2.8 PGM2 Giardia has two proteins with phosphoglucomutase activity, one of which (PGM2) also has phosphomannomutase activity Giardia intestinalis