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Literature summary extracted from

  • Forcella, M.; Cardona, F.; Goti, A.; Parmeggiani, C.; Cipolla, L.; Gregori, M.; Schirone, R.; Fusi, P.; Parenti, P.
    A membrane-bound trehalase from Chironomus riparius larvae: purification and sensitivity to inhibition (2010), Glycobiology, 20, 1186-1195.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine
-
 Chironomus riparius
3.2.1.28 (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine
-
 Sus scrofa
3.2.1.28 (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine
-
 Chironomus riparius
3.2.1.28 (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine
-
 Sus scrofa
3.2.1.28 7-deoxycasuarine
-
 Chironomus riparius
3.2.1.28 7-deoxycasuarine
-
 Sus scrofa
3.2.1.28 7-homocasuarine
-
 Chironomus riparius
3.2.1.28 7-homocasuarine
-
 Sus scrofa
3.2.1.28 castanospermine
-
 Chironomus riparius
3.2.1.28 casuarine
-
 Chironomus riparius
3.2.1.28 deoxynojirimycin inhibition according to a ligand exclusion model Chironomus riparius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.44
-
 alpha,alpha-trehalose pH 5.5, 30°C Chironomus riparius
3.2.1.28 0.48
-
 alpha,alpha-trehalose pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.52
-
 alpha,alpha-trehalose pH 7.5, 30°C Chironomus riparius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 67000
-
 gel filtration, PAGE Chironomus riparius

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Chironomus riparius
-
-
-
3.2.1.28 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Chironomus riparius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 kidney
-
 Sus scrofa
-
3.2.1.28 larva
-
 Chironomus riparius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 75
-
 pH 6.5, 30°C Sus scrofa
3.2.1.28 75
-
 pH 6.5, 30°C Chironomus riparius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Sus scrofa 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Chironomus riparius 2 D-glucose
-
 ?
3.2.1.28 additional information very poor substrates: lactose 0.1%, sucrose 0.08%, cellobiose 0.05%, maltose 0% and raffinose 0.9% of activity against trehalose, respectively Chironomus riparius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 monomer 1 * 67000, SDS-PAGE Chironomus riparius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6.5
-
-
 Chironomus riparius

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.28 0.00012
-
 casuarine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.00059
-
 7-deoxycasuarine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.00139
-
 deoxynojirimycin pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.00486
-
 castanospermine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.0053
-
 (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine pH 6.5, 30°C Sus scrofa
3.2.1.28 0.0093
-
 (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.0117
-
 7-deoxycasuarine pH 6.5, 30°C Sus scrofa
3.2.1.28 0.0256
-
 (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.033
-
 7-homocasuarine pH 6.5, 30°C Chironomus riparius
3.2.1.28 0.0554
-
 (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine pH 6.5, 30°C Sus scrofa
3.2.1.28 0.107
-
 7-homocasuarine pH 6.5, 30°C Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.28 0.00025
-
 pH 6.5, 30°C Chironomus riparius casuarine
3.2.1.28 0.0012
-
 pH 6.5, 30°C Chironomus riparius 7-deoxycasuarine
3.2.1.28 0.00283
-
 pH 6.5, 30°C Chironomus riparius deoxynojirimycin
3.2.1.28 0.00993
-
 pH 6.5, 30°C Chironomus riparius castanospermine
3.2.1.28 0.0106
-
 pH 6.5, 30°C Sus scrofa (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine
3.2.1.28 0.019
-
 pH 6.5, 30°C Chironomus riparius (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine
3.2.1.28 0.0215
-
 pH 6.5, 30°C Sus scrofa 7-deoxycasuarine
3.2.1.28 0.0524
-
 pH 6.5, 30°C Chironomus riparius (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine
3.2.1.28 0.0655
-
 pH 6.5, 30°C Chironomus riparius 7-homocasuarine
3.2.1.28 0.1108
-
 pH 6.5, 30°C Sus scrofa (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine
3.2.1.28 0.214
-
 pH 6.5, 30°C Sus scrofa 7-homocasuarine