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Literature summary extracted from

  • Paulander, W.; Andersson, D.I.; Maisnier-Patin, S.
    Amplification of the gene for isoleucyl-tRNA synthetase facilitates adaptation to the fitness cost of mupirocin resistance in Salmonella enterica (2010), Genetics, 185, 305-312.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.5 gene ileS, quantitative real-time PCR expression analysis Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.5 P184T naturally occuring mutation that restores fitness in mupirocin resistant strains Salmonella enterica
6.1.1.5 Q420H naturally occuring mutation that restores fitness in mupirocin resistant strains Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.5 mupirocin Mup, an isoleucyl-adenylate analogue that inhibits the essential enzyme, isoleucyl-tRNA synthetase Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Salmonella enterica
-
 gene ileS
-

Synonyms

EC Number Synonyms Comment Organism
6.1.1.5 IleRS
-
 Salmonella enterica
6.1.1.5 Isoleucyl-tRNA synthetase
-
 Salmonella enterica

General Information

EC Number General Information Comment Organism
6.1.1.5 additional information in mupirocin-resistant strains, e.g. evolved strain C12 that carried several copies of ileS, the antibiotic resistance leads also to reduced growth rates, these can be restored by the organism via increased expression of the original mutant ileS gene, also improving fitness while maintaining resistance, a process of adaptation initiated by common amplifications and followed by later acquisition of rare point mutations. A point mutation in one copy relaxes selection and allows loss of defective ileS copies, overview. Model for genetic adaptation of cells to the growth limitation caused by their MupR, overview Salmonella enterica