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Literature summary extracted from

  • Jang, M.; Lee, W.; Shin, S.; Han, N.; Seo, J.; Kim, M.
    Molecular cloning of the genes for GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase from Bacteroides thetaiotaomicron (2010), Food Sci. Biotechnol., 19, 849-855.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.47 GMD sequence determination and analysis, sequence comparison and phylogenetic tree, functional overexpression of soluble GMD in Escherichia coli strain BL21(DE3) from an IPTG-inducible expression vector, optimum expression conditions are 30°C and 0.1 mM IPTG Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.47 additional information Bacteroides thetaiotaomicron co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose ?
-
 ?
4.2.1.47 additional information Bacteroides thetaiotaomicron VPI-5482 co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Bacteroides thetaiotaomicron
-
 ATCC 29148D-5
-
4.2.1.47 Bacteroides thetaiotaomicron VPI-5482
-
 ATCC 29148D-5
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 additional information co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose Bacteroides thetaiotaomicron ?
-
 ?
4.2.1.47 additional information co-expression and activities of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthetase lead toptoduction of GDP-L-fucose Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.47 GDP-mannose 4, 6-dehydratase
-
 Bacteroides thetaiotaomicron
4.2.1.47 GMD
-
 Bacteroides thetaiotaomicron

General Information

EC Number General Information Comment Organism
4.2.1.47 evolution GMD belongs to the reductase-epimerase-dehydrogenase superfamily which has a catalytic triad, Ser-Tyr-Lys, that is highly conserved Bacteroides thetaiotaomicron
4.2.1.47 physiological function GMD is involved in the breakdown of ingested polysaccharides into a form that could not be absorbed and utilized by the host otherwise Bacteroides thetaiotaomicron