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Literature summary extracted from
- Gene cloning, expression, and characterization of glucose-1-phosphatase from Enterobacter cloacae B11 (2010), Fish. Aqua. Sci., 13, 49-55.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.10 | gene AgpEnB11, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Enterobacter cloacae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.10 | Cu2+ | - |
Enterobacter cloacae |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.10 | intracellular | - |
Enterobacter cloacae | 5622 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.10 | Ca2+ | activates | Enterobacter cloacae | |
| 3.1.3.10 | Co2+ | activates | Enterobacter cloacae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 47000 | - |
x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Enterobacter cloacae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.10 | alpha-D-glucose 1-phosphate + H2O | Enterobacter cloacae | high activity | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 1-phosphate + H2O | Enterobacter cloacae B11 | high activity | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 6-phosphate + H2O | Enterobacter cloacae | high activity | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 6-phosphate + H2O | Enterobacter cloacae B11 | high activity | D-glucose + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.10 | Enterobacter cloacae | - |
isolate from seawater, gene AgpEnB11 | - |
| 3.1.3.10 | Enterobacter cloacae B11 | - |
isolate from seawater, gene AgpEnB11 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.10 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Enterobacter cloacae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.10 | alpha-D-glucose 1-phosphate + H2O | high activity | Enterobacter cloacae | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 1-phosphate + H2O | high activity | Enterobacter cloacae B11 | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 6-phosphate + H2O | high activity | Enterobacter cloacae | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | alpha-D-glucose 6-phosphate + H2O | high activity | Enterobacter cloacae B11 | D-glucose + phosphate | - |
? | |
| 3.1.3.10 | additional information | the enzyme hydrolyzes a wide variety of phosphorylated compounds, primarily small monosaccharide phosphates, overview | Enterobacter cloacae | ? | - |
? | |
| 3.1.3.10 | additional information | the enzyme hydrolyzes a wide variety of phosphorylated compounds, primarily small monosaccharide phosphates, overview | Enterobacter cloacae B11 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.10 | ? | x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Enterobacter cloacae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.10 | acid glucose-1-phosphatase | - |
Enterobacter cloacae |
| 3.1.3.10 | AGP | - |
Enterobacter cloacae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 50 | - |
recombinant His-tagged enzyme | Enterobacter cloacae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 30 | 70 | activity profile, overview | Enterobacter cloacae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 30 | 70 | stability profile, overview | Enterobacter cloacae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 5 | - |
recombinant His-tagged enzyme | Enterobacter cloacae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 2 | 10 | activity profile, overview | Enterobacter cloacae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.10 | 2 | 10 | stability profile, overview | Enterobacter cloacae |
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