EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.35 | additional information | addition of MgCl2, CaCl2, and EDTA shows no significant effect | Talaromyces purpureogenus | |
3.2.1.35 | NaCl | 0.1-0.4 M NaCl increases the enzyme's activity by 20-30% | Talaromyces purpureogenus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.35 | expression in Escherichia coli | Talaromyces purpureogenus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 30000 | - |
PAGE | Talaromyces purpureogenus |
3.2.1.35 | 31700 | - |
1 * 30000, SDS-PAGE, 1 * 31700, calculated | Talaromyces purpureogenus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.35 | Talaromyces purpureogenus | E7FI87 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.35 | native enzyme | Talaromyces purpureogenus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.35 | culture supernatant | - |
Talaromyces purpureogenus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.35 | hyaluronan + H2O | - |
Talaromyces purpureogenus | ? | catalyzes the endolytic hydrolysis of beta-1,4 glycosidic linkages in hyaluronan. Products are saturated hyaluronan-disaccharides | ? | |
3.2.1.35 | additional information | no substrate: chondroitin | Talaromyces purpureogenus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.35 | monomer | 1 * 30000, SDS-PAGE, 1 * 31700, calculated | Talaromyces purpureogenus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 43 | - |
- |
Talaromyces purpureogenus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 37 | 46 | more than 80% of maximum activity within | Talaromyces purpureogenus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 50 | - |
1 h, pH 3.0, more than 95% of initial activity | Talaromyces purpureogenus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 3 | 3.3 | - |
Talaromyces purpureogenus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.35 | 4.3 | - |
no activity above | Talaromyces purpureogenus |