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Literature summary extracted from

  • Takahashi, K.; Tagami, U.; Shimba, N.; Kashiwagi, T.; Ishikawa, K.; Suzuki, E.
    Crystal structure of Bifidobacterium longum phosphoketolase; key enzyme for glucose metabolism in Bifidobacterium (2010), FEBS Lett., 584, 3855-3861.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.22 using 10 mg/ml thiamine diphosphate, 4% (v/v) Tacsimate pH 5.0, 12% (w/v) polyethylene glycol 3350 for the native enzyme. Se-Met substituted protein crystals are grown with 17% (w/v) polyethylene glycol 3350, 0.1 M MES (pH6.6), 0.2 M sodium thiocyanate in the absence of thiamine diphosphate Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.22 Ca2+ contains Ca2+ bound to the active site Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.22 92500
-
 2 * 92500, SDS-PAGE Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.22 Bifidobacterium longum
-
-
-
4.1.2.22 Bifidobacterium longum JCM 1217
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.22 HisTrap column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
 Bifidobacterium longum Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
 Bifidobacterium longum JCM 1217 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-xylulose 5-phosphate + phosphate
-
 Bifidobacterium longum acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.22 D-xylulose 5-phosphate + phosphate
-
 Bifidobacterium longum JCM 1217 acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.22 homodimer 2 * 92500, SDS-PAGE Bifidobacterium longum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.22 thiamine diphosphate dependent on Bifidobacterium longum