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Literature summary extracted from

  • Wyman, S.; Simpson, R.J.; McKie, A.T.; Sharp, P.A.
    Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro (2008), FEBS Lett., 582, 1901-1906.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.1.3 dehydroascorbate preloading cells with dehydroascorbate greatly increases both ferric and cupric reductase activities of Dcytb Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.3 Dcytb-EGFP is expressed in tetracycline-off Madin-Darby canine kidney cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.1.3 0.0152
-
 Cu(II)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C Mus musculus
7.2.1.3 0.0231
-
 Cu(II)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C Mus musculus
7.2.1.3 0.074
-
 Fe(III)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 37°C Mus musculus
7.2.1.3 0.0921
-
 Fe(III)-nitrilotriacetic acid[side 2] in 25 mM MOPS, 25 mM MES, 5.4 mM KCl, 5 mM glucose, 140 mM NaCl, 1.8 mM CaCl2, 0.8 mM MgCl2, pH 7.0, at 22°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.3 plasma membrane
-
 Mus musculus 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.3 ascorbate[side 1] + Fe(III)[side 2] Mus musculus
-
 monodehydroascorbate[side 1] + Fe(II)[side 2]
-
 ?
7.2.1.3 cupric-histidine[side 1] + ? Mus musculus
-
 ?
-
 ?
7.2.1.3 ferric citrate[side 1] + ? Mus musculus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.3 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.1.3 duodenum
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.3 ascorbate[side 1] + Fe(III)[side 2]
-
 Mus musculus monodehydroascorbate[side 1] + Fe(II)[side 2]
-
 ?
7.2.1.3 bathocuprionedisulfonate[side 1] + Cu(II)-nitrilotriacetic acid[side 2]
-
 Mus musculus Cu(I)-bathocuproinedisulfonate
-
 ?
7.2.1.3 cupric-histidine[side 1] + ?
-
 Mus musculus ?
-
 ?
7.2.1.3 ferric citrate[side 1] + ?
-
 Mus musculus ?
-
 ?
7.2.1.3 ferrozine[side 1] + Fe(III)-nitrilotriacetic acid[side 2]
-
 Mus musculus Fe(II)-ferrozine
-
 ?
7.2.1.3 additional information Dcytb has the capacity to reduce both iron and copper complexes Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.3 Cybrd1
-
 Mus musculus
7.2.1.3 DCytb
-
 Mus musculus
7.2.1.3 duodenal cytochrome b
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.3 ascorbate dependent on Mus musculus

Expression

EC Number Organism Comment Expression
7.2.1.3 Mus musculus expression of Dcytb-EGFP is knocked down by addition of 20 ng/ml doxycycline down

General Information

EC Number General Information Comment Organism
7.2.1.3 physiological function Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter1 and copper transporter 1, respectively Mus musculus