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Literature summary extracted from

  • Hundle, B.; OBrien, D.; Beyer, P.; Kleining, H.; Hearst, J.
    In vitro expression and activity of lycopene cyclase and beta-carotene hydroxylase from Erwinia herbicola (1993), FEBS Lett., 315, 329-334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.24 expressed in Escherichia coli BL21(DE3) cells Pantoea agglomerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.24 cytoplasm exclusively located in the cytoplasm Pantoea agglomerans 5737
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.24 22000
-
 SDS-PAGE Pantoea agglomerans

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.24 Pantoea agglomerans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
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 Pantoea agglomerans beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
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 Pantoea agglomerans zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.24 beta-carotene hydroxylase
-
 Pantoea agglomerans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.24 additional information the beta-carotene hydroxylase reaction proceeds in the absence of externally added cofactors. However, electron donors, such as NAD, NADPH, and ascorbate were able to stimulate the reaction Pantoea agglomerans