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Literature summary extracted from

  • Cenas, N.; Bironaite, D.; Kulys, J.
    On the mechanism of rotenone-insensitive reduction of quinones by mitochondrial NADH:ubiquinone reductase. The high affinity binding of NAD+ and NADH to the reduced enzyme form (1991), FEBS Lett., 284, 192-194.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 ADP-ribose ADP-ribose acts as mixed-type inhibitors for NADH, ferricyanide and 5,8-dioxy-1,4-naphthoquinone Bos taurus
7.1.1.2 arylazido-beta-alanyl-NAD+ competitive inhibition towards NADH and ferricyanide Bos taurus
7.1.1.2 additional information the oxidation of NADH by 5,8-dioxy-1,4-naphthoquinone, catalyzed by complex I is completely insensitive to 0.002 mM rotenone Bos taurus
7.1.1.2 NAD+ NAD+ acts as mixed-type inhibitors for NADH, ferricyanide and 5,8-dioxy-1,4-naphthoquinone. At saturating concentrations of oxidizer, NAD+ acts as a competitive inhibitor for NADH Bos taurus
7.1.1.2 NADH NADH acts as a competitive inhibitor for 5,8-dioxy-1,4-naphthoquinone, NADH acts as a linear competitive inhibitor for ferricyanide Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 2 ferricyanide + NADH
-
 Bos taurus 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 NADH + H+ + 5,8-dioxo-1,4-naphthoquinone
-
 Bos taurus NAD+ + 5,8-dioxo-1,4-naphthoquinol
-
 ?
7.1.1.2 NADH + H+ + ubiquinone
-
 Bos taurus NAD+ + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN
-
 Bos taurus
7.1.1.2 iron-sulfur centre
-
 Bos taurus
7.1.1.2 NADH
-
 Bos taurus
7.1.1.2 ubiquinone
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.1.1.2 0.012
-
 NADH in 0.4 M phosphate, using 5,8-dioxy-1,4-naphthoquinone as substrate, at pH 7.0, 25°C Bos taurus
7.1.1.2 0.05
-
 NADH in 0.4 M phosphate, using ferricyanide as substrate, at pH 7.0, 25°C Bos taurus