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Literature summary extracted from

  • Bharath, S.R.; Bisht, S.; Savithri, H.S.; Murthy, M.R.
    Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium - implications on substrate specificity and catalysis (2011), FEBS J., 278, 2879-2891.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.18 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.18 both wild-type and selenomethionine labelled holoenzyme, to 2.4 and 1.9 A resolution, respectively. Significant electron density is not observed for the cofactor pyridoxal 5'-phosphate. Wild-type enzyme is in an open conformation while selenomethionine enzyme, crystallized in the presence of isoserine, is in a closed conformation suggesting that the enzyme is likely to undergo conformational changes upon binding of substrate. Thr166 may be involved in abstraction of proton from the Calpha atom of the substrate Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.18 0.42
-
 D-Ser pH 7.5, 37°C, buffer K+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 0.45
-
 D-Thr pH 7.5, 37°C, buffer Na+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 0.53
-
 D-Thr pH 7.5, 37°C, buffer K+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 0.63
-
 D-allo-Thr pH 7.5, 37°C, buffer Na+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 0.87
-
 D-Ser pH 7.5, 37°C, buffer Na+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 0.88
-
 D-allo-Thr pH 7.5, 37°C, buffer K+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 10.2
-
 L-Ser pH 7.5, 37°C, buffer K+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 11.8
-
 L-Ser pH 7.5, 37°C, buffer Na+-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.18 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.18 recombinant protein Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.18 2.09
-
 wild-type, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 2.12
-
 selenomethionine labelled enzyme, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.18 D-allo-Thr
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutanoate + NH3
-
 ?
4.3.1.18 D-Ser
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.3.1.18 D-Thr
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutanoate + NH3
-
 ?
4.3.1.18 L-Ser
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ir

Synonyms

EC Number Synonyms Comment Organism
4.3.1.18 DSD
-
 Salmonella enterica subsp. enterica serovar Typhimurium