BRENDA - Enzyme Database

Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells

Cruz, A.H.; Brock, M.; Zambuzzi-Carvalho, P.F.; Santos-Silva, L.K.; Troian, R.F.; Goes, A.M.; Soares, C.M.; Pereira, M.; FEBS J. 278, 2318-2332 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
a thioredoxin/His-tagged version expressed in Escherichia coli BL21(DE3)
Paracoccidioides brasiliensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
60000
-
x * 60000, SDS-PAGE
Paracoccidioides brasiliensis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Paracoccidioides brasiliensis
Q5YLB6
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
phosphoprotein
inactivation by phosphorylation
Paracoccidioides brasiliensis
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
immobilized metal ion affinity chromatography (Ni2+), tags removed
Paracoccidioides brasiliensis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
3.85
-
pH and temperature not specified in the publication
Paracoccidioides brasiliensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
714980
Paracoccidioides brasiliensis
glyoxylate + succinate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
?
x * 60000, SDS-PAGE
Paracoccidioides brasiliensis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.3.1
Paracoccidioides brasiliensis
predicted
-
6.79
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
a thioredoxin/His-tagged version expressed in Escherichia coli BL21(DE3)
Paracoccidioides brasiliensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
60000
-
x * 60000, SDS-PAGE
Paracoccidioides brasiliensis
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.3.1
phosphoprotein
inactivation by phosphorylation
Paracoccidioides brasiliensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
immobilized metal ion affinity chromatography (Ni2+), tags removed
Paracoccidioides brasiliensis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
3.85
-
pH and temperature not specified in the publication
Paracoccidioides brasiliensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
714980
Paracoccidioides brasiliensis
glyoxylate + succinate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
?
x * 60000, SDS-PAGE
Paracoccidioides brasiliensis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.3.1
Paracoccidioides brasiliensis
predicted
-
6.79
General Information
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
glyoxylate cycle key enzyme
Paracoccidioides brasiliensis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
glyoxylate cycle key enzyme
Paracoccidioides brasiliensis