Literature summary extracted from
Espariz, M.; Repizo, G.; Blancato, V.; Mortera, P.; Alarcon, S.; Magni, C.
Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis (2011), FEBS J., 278, 2140-2151.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.112 |
expressed in Escherichia coli BL21(DE3) cells |
Enterococcus faecalis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
ADP |
78% residual activity at 0.25 mM |
Enterococcus faecalis |
|
4.1.1.112 |
ATP |
37% residual activity at 1 mM |
Enterococcus faecalis |
|
4.1.1.112 |
citrate |
74% residual activity at 2 mM |
Enterococcus faecalis |
|
4.1.1.112 |
EDTA |
complete inhibition at 2 mM |
Enterococcus faecalis |
|
4.1.1.112 |
malate |
65% residual activity at 2 mM |
Enterococcus faecalis |
|
4.1.1.112 |
malonate |
26% residual activity at 2 mM |
Enterococcus faecalis |
|
4.1.1.112 |
additional information |
not inhibited by succinate |
Enterococcus faecalis |
|
4.1.1.112 |
NAD+ |
40% residual activity at 0.25 mM |
Enterococcus faecalis |
|
4.1.1.112 |
NADH |
53% residual activity at 0.25 mM |
Enterococcus faecalis |
|
4.1.1.112 |
oxalate |
14% residual activity at 2 mM |
Enterococcus faecalis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
0.62 |
- |
oxaloacetate |
calculated value at pH 4.5, temperature not specified in the publication |
Enterococcus faecalis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
Mn2+ |
the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+ |
Enterococcus faecalis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.112 |
Enterococcus faecalis |
Q82YW6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.1.112 |
Ni2+-bounded affinity column chromatography |
Enterococcus faecalis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.112 |
Oxaloacetate |
- |
Enterococcus faecalis |
Pyruvate + CO2 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.112 |
CitM |
- |
Enterococcus faecalis |
4.1.1.112 |
OAD |
- |
Enterococcus faecalis |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
11.2 |
- |
oxaloacetate |
calculated value at pH 4.5, temperature not specified in the publication |
Enterococcus faecalis |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.1.112 |
4.5 |
- |
- |
Enterococcus faecalis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.1.1.112 |
3.5 |
5 |
- |
Enterococcus faecalis |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
additional information |
no nicotinamide cofactors required |
Enterococcus faecalis |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.1.1.112 |
malfunction |
the citrate fermentation phenotype is not affected by citM deletion |
Enterococcus faecalis |
4.1.1.112 |
metabolism |
CitM is not required for efficient citrate utilization |
Enterococcus faecalis |
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.112 |
22.3 |
- |
oxaloacetate |
calculated value at pH 4.5, temperature not specified in the publication |
Enterococcus faecalis |
|