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Literature summary extracted from

  • Kneen, M.; Stan, R.; Yep, A.; Tyler, R.; Saehuan, C.; McLeish, M.
    Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae (2011), FEBS J., 278, 1842-1853.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.43 expressed in Escherichia coli BL21(DE3)pLysS cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.43 E545L the mutant shows 730fold decrease in kcat (phenylpyruvate) and 38fold decrease in kcat (indole-3-pyruvate) compared to the wild type enzyme Saccharomyces cerevisiae
4.1.1.43 I335Y compared to the wild type enzyme, the mutant exhibits 0.6% catalytic efficiency with phenylpyruvate, 33% catalytic efficiency with 2-oxohexanoic acid, 213% catalytic efficiency with 2-oxopentanoic acid, 1.7% catalytic efficiency with 2-oxobutanoic acid, and 7% catalytic efficiency with pyruvic acid Saccharomyces cerevisiae
4.1.1.43 I335Y/M624W compared to the wild type enzyme, the mutant exhibits 0.02% catalytic efficiency with phenylpyruvate, 2.3% catalytic efficiency with 2-oxohexanoic acid, 25% catalytic efficiency with 2-oxopentanoic acid, no activity with 2-oxobutanoic acid, and 59% catalytic efficiency with pyruvic acid Saccharomyces cerevisiae
4.1.1.43 M624W compared to the wild type enzyme, the mutant exhibits 3.4% catalytic efficiency with phenylpyruvate, 32% catalytic efficiency with 2-oxohexanoic acid, 131% catalytic efficiency with 2-oxopentanoic acid, 1.4% catalytic efficiency with 2-oxobutanoic acid, and 152% catalytic efficiency with pyruvic acid Saccharomyces cerevisiae
4.1.1.43 Q448W compared to the wild type enzyme, the mutant exhibits 45% catalytic efficiency with phenylpyruvate, 16% catalytic efficiency with 2-oxohexanoic acid, 231% catalytic efficiency with 2-oxopentanoic acid, 62% catalytic efficiency with 2-oxobutanoic acid, and 130% catalytic efficiency with pyruvic acid Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.43 0.03
-
Indole-3-pyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.054
-
phenylpyruvate mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.09
-
4-hydroxyphenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.09
-
2-oxo-4-phenylbutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.1
-
phenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.17
-
2-oxohexanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.18
-
phenylpyruvate mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.37
-
2-oxopentanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.46
-
2-oxohexanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.54
-
2-oxopentanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.55
-
2-oxohexanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.63
-
2-oxopentanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.64
-
4-methylthio-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.69
-
2-oxohexanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.8
-
phenylpyruvate mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.9
-
4-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.96
-
2-oxopentanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 1
-
2-oxohexanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.1
-
2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.1
-
3-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 5.5
-
2-oxobutanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 5.5
-
Pyruvic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 6.4
-
2-oxobutanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 6.4
-
Pyruvic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 7
-
2-oxobutanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 7.6
-
2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 8.5
-
3-methyl-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 9.7
-
Pyruvic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 25
-
Pyruvic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.43 57000
-
x * 57000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.43 Saccharomyces cerevisiae Q06408
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.43 Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.43 2-oxo-4-phenylbutanoic acid 9% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 2-oxobutanoic acid 0.2% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 2-oxohexanoic acid 6% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 2-oxopentanoic acid 1% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 3-methyl-2-oxobutanoic acid 1% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 3-methyl-2-oxopentanoic acid 2% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 4-hydroxyphenylpyruvate 63% activity compared to phenylpyruvate Saccharomyces cerevisiae 4-hydroxyphenylacetaldehyde + CO2
-
?
4.1.1.43 4-methyl-2-oxopentanoic acid 6% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 4-methylthio-2-oxobutanoic acid 6% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 benzoylformic acid 0.2% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?
4.1.1.43 indole-3-pyruvate preferred substrate, 100% activity Saccharomyces cerevisiae indoleacetaldehyde + CO2
-
?
4.1.1.43 additional information PPDC is essentially unable to utilize pyruvate and 3-indoleglyoxylic acid Saccharomyces cerevisiae ?
-
?
4.1.1.43 Phenylpyruvate preferred substrate, 100% activity Saccharomyces cerevisiae Phenylacetaldehyde + CO2
-
?
4.1.1.43 pyruvic acid 0.02% activity compared to phenylpyruvate Saccharomyces cerevisiae ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.43 ? x * 57000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
4.1.1.43 phenylpyruvate decarboxylase
-
Saccharomyces cerevisiae
4.1.1.43 PPDC
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.43 0.04
-
2-oxobutanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.06
-
2-oxobutanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.064
-
Pyruvic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.14
-
Indole-3-pyruvate mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.32
-
Pyruvic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.34
-
Pyruvic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.39
-
2-oxopentanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.41
-
2-oxohexanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.45
-
Pyruvic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.76
-
2-oxopentanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.85
-
phenylpyruvate mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.96
-
2-oxohexanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 1.2
-
phenylpyruvate mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 1.3
-
2-oxohexanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 1.7
-
2-oxo-4-phenylbutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2
-
2-oxopentanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.1
-
2-oxopentanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.2
-
2-oxobutanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.3
-
2-oxohexanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.9
-
2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 4.8
-
phenylpyruvate mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 5.2
-
2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 5.4
-
Indole-3-pyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 7.7
-
4-methylthio-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 8.8
-
phenylpyruvate mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 8.8
-
2-oxohexanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 10
-
4-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 11
-
4-hydroxyphenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 11
-
3-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 19
-
3-methyl-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 20
-
phenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.43 6.5 7
-
Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.43 thiamine diphosphate dependent on Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.43 0.003
-
Pyruvic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.007
-
2-oxobutanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.009
-
2-oxobutanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.027
-
Pyruvic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.03
-
phenylpyruvate mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.035
-
Pyruvic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.06
-
Pyruvic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.07
-
Pyruvic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.315
-
2-oxobutanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.35
-
benzoylformic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.4
-
2-oxopentanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.42
-
2-oxohexanoic acid mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 0.52
-
2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 1.1
-
phenylpyruvate mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.1
-
2-oxopentanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.2
-
3-methyl-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.5
-
2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 2.8
-
2-oxohexanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.4
-
2-oxopentanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.5
-
3-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 3.7
-
2-oxopentanoic acid mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 5.8
-
2-oxohexanoic acid mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 6
-
2-oxohexanoic acid mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 11
-
4-methyl-2-oxopentanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 12
-
4-methylthio-2-oxobutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 13
-
2-oxohexanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 19
-
2-oxo-4-phenylbutanoic acid wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 125
-
4-hydroxyphenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 200
-
phenylpyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae
4.1.1.43 200
-
Indole-3-pyruvate wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C Saccharomyces cerevisiae