EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.43 | expressed in Escherichia coli BL21(DE3)pLysS cells | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.43 | E545L | the mutant shows 730fold decrease in kcat (phenylpyruvate) and 38fold decrease in kcat (indole-3-pyruvate) compared to the wild type enzyme | Saccharomyces cerevisiae |
4.1.1.43 | I335Y | compared to the wild type enzyme, the mutant exhibits 0.6% catalytic efficiency with phenylpyruvate, 33% catalytic efficiency with 2-oxohexanoic acid, 213% catalytic efficiency with 2-oxopentanoic acid, 1.7% catalytic efficiency with 2-oxobutanoic acid, and 7% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
4.1.1.43 | I335Y/M624W | compared to the wild type enzyme, the mutant exhibits 0.02% catalytic efficiency with phenylpyruvate, 2.3% catalytic efficiency with 2-oxohexanoic acid, 25% catalytic efficiency with 2-oxopentanoic acid, no activity with 2-oxobutanoic acid, and 59% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
4.1.1.43 | M624W | compared to the wild type enzyme, the mutant exhibits 3.4% catalytic efficiency with phenylpyruvate, 32% catalytic efficiency with 2-oxohexanoic acid, 131% catalytic efficiency with 2-oxopentanoic acid, 1.4% catalytic efficiency with 2-oxobutanoic acid, and 152% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
4.1.1.43 | Q448W | compared to the wild type enzyme, the mutant exhibits 45% catalytic efficiency with phenylpyruvate, 16% catalytic efficiency with 2-oxohexanoic acid, 231% catalytic efficiency with 2-oxopentanoic acid, 62% catalytic efficiency with 2-oxobutanoic acid, and 130% catalytic efficiency with pyruvic acid | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.43 | 0.03 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.054 | - |
phenylpyruvate | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.09 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.09 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.1 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.17 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.18 | - |
phenylpyruvate | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.37 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.46 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.54 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.55 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.63 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.64 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.69 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.8 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.9 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.96 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 1 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.1 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.1 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 5.5 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 5.5 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 6.4 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 6.4 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 7 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 7.6 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 8.5 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 9.7 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 25 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.43 | 57000 | - |
x * 57000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.43 | Saccharomyces cerevisiae | Q06408 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.43 | Ni-NTA column chromatography | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.43 | 2-oxo-4-phenylbutanoic acid | 9% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 2-oxobutanoic acid | 0.2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 2-oxohexanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 2-oxopentanoic acid | 1% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 3-methyl-2-oxobutanoic acid | 1% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 3-methyl-2-oxopentanoic acid | 2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 4-hydroxyphenylpyruvate | 63% activity compared to phenylpyruvate | Saccharomyces cerevisiae | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
4.1.1.43 | 4-methyl-2-oxopentanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | 4-methylthio-2-oxobutanoic acid | 6% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | benzoylformic acid | 0.2% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | indole-3-pyruvate | preferred substrate, 100% activity | Saccharomyces cerevisiae | indoleacetaldehyde + CO2 | - |
? | |
4.1.1.43 | additional information | PPDC is essentially unable to utilize pyruvate and 3-indoleglyoxylic acid | Saccharomyces cerevisiae | ? | - |
? | |
4.1.1.43 | Phenylpyruvate | preferred substrate, 100% activity | Saccharomyces cerevisiae | Phenylacetaldehyde + CO2 | - |
? | |
4.1.1.43 | pyruvic acid | 0.02% activity compared to phenylpyruvate | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.43 | ? | x * 57000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.43 | phenylpyruvate decarboxylase | - |
Saccharomyces cerevisiae |
4.1.1.43 | PPDC | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.43 | 0.04 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.06 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.064 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.14 | - |
Indole-3-pyruvate | mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.32 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.34 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.39 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.41 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.45 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.76 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.85 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.96 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 1.2 | - |
phenylpyruvate | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 1.3 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 1.7 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.1 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.2 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.3 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.9 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 4.8 | - |
phenylpyruvate | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 5.2 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 5.4 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 7.7 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 8.8 | - |
phenylpyruvate | mutant enzyme E545L, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 8.8 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 10 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 11 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 11 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 19 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 20 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.43 | 6.5 | 7 | - |
Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.43 | thiamine diphosphate | dependent on | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.43 | 0.003 | - |
Pyruvic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.007 | - |
2-oxobutanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.009 | - |
2-oxobutanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.027 | - |
Pyruvic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.03 | - |
phenylpyruvate | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.035 | - |
Pyruvic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.06 | - |
Pyruvic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.07 | - |
Pyruvic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.315 | - |
2-oxobutanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.35 | - |
benzoylformic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.4 | - |
2-oxopentanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.42 | - |
2-oxohexanoic acid | mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 0.52 | - |
2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 1.1 | - |
phenylpyruvate | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.1 | - |
2-oxopentanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.2 | - |
3-methyl-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.5 | - |
2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 2.8 | - |
2-oxohexanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.4 | - |
2-oxopentanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.5 | - |
3-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 3.7 | - |
2-oxopentanoic acid | mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 5.8 | - |
2-oxohexanoic acid | mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 6 | - |
2-oxohexanoic acid | mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 11 | - |
4-methyl-2-oxopentanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 12 | - |
4-methylthio-2-oxobutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 13 | - |
2-oxohexanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 19 | - |
2-oxo-4-phenylbutanoic acid | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 125 | - |
4-hydroxyphenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 200 | - |
phenylpyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae | |
4.1.1.43 | 200 | - |
Indole-3-pyruvate | wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C | Saccharomyces cerevisiae |