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Literature summary extracted from

  • Pesi, R.; Allegrini, S.; Careddu, M.G.; Filoni, D.N.; Camici, M.; Tozzi, M.G.
    Active and regulatory sites of cytosolic 5'-nucleotidase (2010), FEBS J., 277, 4863-4872.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 2,3-bisphosphoglycerate
-
 Bos taurus
3.1.3.5 ADP
-
 Bos taurus
3.1.3.5 ATP
-
 Bos taurus
3.1.3.5 diadenosine polyphosphate
-
 Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5 expression of His6-tagged wild-type and mutant enzymes Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.5 D396A site-directed mutagenesis, the mutant is only activable by 2,3-bisphosphoglycerate Bos taurus
3.1.3.5 F127E site-directed mutagenesis Bos taurus
3.1.3.5 F36R site-directed mutagenesis, inactive mutant Bos taurus
3.1.3.5 G319D site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme Bos taurus
3.1.3.5 H428D site-directed mutagenesis Bos taurus
3.1.3.5 I152D site-directed mutagenesis Bos taurus
3.1.3.5 K311A site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme Bos taurus
3.1.3.5 M436W site-directed mutagenesis Bos taurus
3.1.3.5 N154D site-directed mutagenesis, the mutant shows a decrease in the value of K50 for Mg2+ compared to the wild-type enzyme Bos taurus
3.1.3.5 R144E site-directed mutagenesis Bos taurus
3.1.3.5 T56V site-directed mutagenesis Bos taurus
3.1.3.5 Y115A site-directed mutagenesis, the mutant behaves similar to the wild-type enzyme Bos taurus
3.1.3.5 Y55G site-directed mutagenesis Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.04
-
 5'-IMP pH not specified in the publication, temperature not specified in the publication, recombinant mutant K311A Bos taurus
3.1.3.5 0.05
-
 5'-IMP pH not specified in the publication, temperature not specified in the publication, recombinant mutant G319D Bos taurus
3.1.3.5 0.06
-
 5'-IMP pH not specified in the publication, temperature not specified in the publication, recombinant mutant Y115A Bos taurus
3.1.3.5 0.1
-
 5'-IMP pH not specified in the publication, temperature not specified in the publication, recombinant wild-type enzyme and mutant D396A Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ activates Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.5 5'-IMP + H2O Bos taurus
-
 inosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Bos taurus O46411
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-IMP + H2O
-
 Bos taurus inosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 tetramer the active tetramer is formed by interaction between two identical dimers via interface B: this interface contains 28 aminoacid residues, of which 8 make hydrogen bonds Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 cN-II
-
 Bos taurus
3.1.3.5 cytosolic 5'-nucleotidase
-
 Bos taurus