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Literature summary extracted from

  • Borjigin, M.; Martinez, B.; Purohit, S.; de la Rosa, G.; Arenaz, P.; Stec, B.
    Chinese hamster apurinic/apyrimidinic endonuclease (chAPE1) expressed in sf9 cells reveals that its endonuclease activity is regulated by phosphorylation (2010), FEBS J., 277, 4732-4740.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.99.18 expression in Sf9 cell Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.99.18 0.000048
-
 18-mer containing P33-labeled tetrahydrofuran enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C Cricetulus griseus
4.2.99.18 0.000081
-
 18-mer containing P33-labeled tetrahydrofuran enzyme phosphorylated by casein kinase II, pH 7.5, 22°C Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.99.18 35500
-
 x * 35500, SDS-PAGE Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
4.2.99.18 Cricetulus griseus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.99.18 phosphoprotein higher phosphorylation of APE1 in the expression host is correlated to a lower endonuclease activity. The phosphorylation of a higher activity batch of APE1 by casein kinase II decreases the endonuclease activity, and the dephosphorylation of APE1 by lambda phosphatase increases the endonuclease activity Cricetulus griseus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.99.18 recombinant protein Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.18 18-mer containing P33-labeled tetrahydrofuran
-
 Cricetulus griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.99.18 ? x * 35500, SDS-PAGE Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.99.18 0.58
-
 18-mer containing P33-labeled tetrahydrofuran enzyme phosphorylated by casein kinase II, pH 7.5, 22°C Cricetulus griseus
4.2.99.18 5.7
-
 18-mer containing P33-labeled tetrahydrofuran enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C Cricetulus griseus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.99.18 7200
-
 18-mer containing P33-labeled tetrahydrofuran enzyme phosphorylated by casein kinase II, pH 7.5, 22°C Cricetulus griseus
4.2.99.18 12000
-
 18-mer containing P33-labeled tetrahydrofuran enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C Cricetulus griseus