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Literature summary extracted from

  • Nikolaeva, O.; Takahashi, Y.; Moiseyev, G.; Ma, J.X.
    Purified RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane (2009), FEBS J., 276, 3020-3030.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.64 expressed in 293A-LRAT cells Gallus gallus

General Stability

EC Number General Stability Organism
3.1.1.64 0.1% (w/v) CHAPS is optimal for solubilizing RPE65 while preserving its enzymatic activity Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.64 CHAPS the addition of 0.5% (w/v) CHAPS to the assay system almost completely abolishes the 11-cis-retinol formation Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.64 0.0037
-
 all-trans-retinyl palmitate purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.64 membrane
-
 Gallus gallus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.64 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.64 Ni-NTA column chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.64 retinal pigment epithelium
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.64 all-trans-retinyl ester + H2O all-trans-retinyl ester must be incorporated into the phospholipid membrane to serve as a substrate for isomerohydrolase RPE65 Gallus gallus 11-cis-retinol + a fatty acid
-
 ?
3.1.1.64 all-trans-retinyl palmitate + H2O high affinity substrate Gallus gallus 11-cis-retinol + palmitate
-
 ?
3.1.1.64 additional information neither retinyl ester nor 11-cis-retinol is produced after incubation of all-trans-retinol with the purified RPE65 (not an intrinsic substrate for RPE65) Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.64 Rpe65
-
 Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.64 0.000145
-
 all-trans-retinyl palmitate purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C Gallus gallus

General Information

EC Number General Information Comment Organism
3.1.1.64 physiological function RPE65 is the isomerohydrolase of the visual cycle Gallus gallus