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Literature summary extracted from

  • Olejnik, K.; Murcha, M.W.; Whelan, J.; Kraszewska, E.
    Cloning and characterization of AtNUDT13, a novel mitochondrial Arabidopsis thaliana Nudix hydrolase specific for long-chain diadenosine polyphosphates (2007), FEBS J., 274, 4877-4885.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.60 dithiothreitol the enzyme requires reducing conditions for optimal activity. The omission of dithiothreitol from the enzymatic reactions reduces its activity by 70% Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.60 overexpressed in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.60 fluoride
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.60 0.61
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.60 mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.60 Mg2+ optimal activity with 5 mM Mg2+. No activity with Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.60 26000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Arabidopsis thaliana
-
 adenosine 5'-tetraphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.60 Arabidopsis thaliana Q52K88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.60
-
 Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP (2) Arabidopsis thaliana
a
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-tetraphosphate + ADP (1) Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.60 additional information diadenosine tetraphosphate and diadenosine triphosphate, adenosine nucleotides, diphosphoinositol polyphosphate and phosphoribosyl pyrophosphate are not hydrolyzed Arabidopsis thaliana ?
-
 ?
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O P1,P5-bis(5'-adenosyl)pentaphosphate is hydrolyzed at 40% of the activity compared to P1,P6-bis(5'-adenosyl)hexaphosphate Arabidopsis thaliana adenosine 5'-tetraphosphate + AMP
-
 ?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
-
 Arabidopsis thaliana adenosine 5'-tetraphosphate + ADP
-
 ?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O preferred substrate. Hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate with the formation of ADP or AMP Arabidopsis thaliana adenosine 5'-tetraphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.60 monomer 1 * 26000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.1.60 At3g26690
-
 Arabidopsis thaliana
3.6.1.60 AtNUDT13
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.60 37
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.60 9.8
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.60 7
-
 assay at Arabidopsis thaliana
3.6.1.60 8.5
-
-
 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.60 0.07
-
 pH 7.6, 37°C Arabidopsis thaliana fluoride

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.60 16
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana