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Literature summary extracted from

  • Zhou, Y.; Shaw, N.; Li, Y.; Zhao, Y.; Zhang, R.; Liu, Z.J.
    Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules (2010), FASEB J., 24, 4668-4677.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.2 mutant C65A is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.2 mutant C65A in complex with oleic acid and palmitoleic acid, hanging drop vapor diffusion method, using 0.1 M citric acid (pH 4.0) and 1.7 M ammonium sulfate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.2 C65A inactive Homo sapiens
5.3.99.2 F83A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
5.3.99.2 K59A the mutation enhances the catalytic efficiency by more than 2fold Homo sapiens
5.3.99.2 L131A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
5.3.99.2 L79A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
5.3.99.2 M64A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
5.3.99.2 M94A the mutation reduces the catalytic efficiency Homo sapiens
5.3.99.2 S45A the mutation reduces the catalytic efficiency Homo sapiens
5.3.99.2 S81A the mutation reduces the catalytic efficiency Homo sapiens
5.3.99.2 T147A the mutation slightly enhances the catalytic efficiency Homo sapiens
5.3.99.2 W54A the mutation reduces the catalytic efficiency Homo sapiens
5.3.99.2 Y149A the mutation enhances the catalytic efficiency by more than 2fold Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.2 0.0102
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0123
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0127
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0138
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0154
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0161
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0202
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0204
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0245
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0252
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0325
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.0328
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.2 Homo sapiens P41222
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.2 Ni-affinity column chromatography and Superdex G75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.99.2 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.2 Prostaglandin H2
-
 Homo sapiens Prostaglandin D2
-
 ir

Subunits

EC Number Subunits Comment Organism
5.3.99.2 monomer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.2 L-PGDS
-
 Homo sapiens
5.3.99.2 L-prostaglandin D synthase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.99.2 0.06
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.63
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.81
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 0.87
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2.23
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2.24
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2.4
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2.64
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2.91
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 3.67
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 5.73
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 5.84
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.99.2 110
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 1170
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 1890
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 2120
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 6570
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 6660
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 8940
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 9420
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 11490
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 14290
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 24840
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.3.99.2 27050
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens