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Literature summary extracted from

  • Kengen, S.W.; Bikker, F.J.; Hagen, W.R.; de Vos, W.M.; van der Oost, J.
    Characterization of a catalase-peroxidase from the hyperthermophilic archaeon Archaeoglobus fulgidus (2001), Extremophiles, 5, 323-332.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21(DE3) cells Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 3-Amino-1,2,4-triazole
-
Archaeoglobus fulgidus
1.11.1.21 azide inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus
1.11.1.21 cyanide inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus
1.11.1.21 H2O2 at a H2O2 concentration of 1 mM, a half-life of inactivation of approximately 60 s is observed, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) protects the enzyme from inactivation by H2O2 Archaeoglobus fulgidus
1.11.1.21 hydroxylamine inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.85
-
H2O2 peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 84900
-
2 * 84900, calculated from amino acid sequence Archaeoglobus fulgidus
1.11.1.21 85000
-
2 * 85000, SDS-PAGE Archaeoglobus fulgidus
1.11.1.21 169000
-
gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Archaeoglobus fulgidus O28050
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 0.45
-
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus
1.11.1.21 23.3
-
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus
1.11.1.21 2375
-
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C Archaeoglobus fulgidus
1.11.1.21 6922
-
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Archaeoglobus fulgidus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
1.11.1.21 2,4-dichlorophenol + H2O2
-
Archaeoglobus fulgidus ?
-
?
1.11.1.21 3,3'-diaminobenzidine + H2O2
-
Archaeoglobus fulgidus oxidized 3,3'-diaminobenzidine + H2O
-
?
1.11.1.21 3,3'-dimethoxybenzidine + H2O2
-
Archaeoglobus fulgidus oxidized 3,3'-dimethoxybenzidine + H2O
-
?
1.11.1.21 4-phenylenediamine + H2O2
-
Archaeoglobus fulgidus oxidized 4-phenylenediamine + H2O
-
?
1.11.1.21 aminoantipyrine + H2O2
-
Archaeoglobus fulgidus oxidized aminoantipyrine + H2O
-
?
1.11.1.21 H2O2
-
Archaeoglobus fulgidus O2 + H2O
-
?
1.11.1.21 additional information no activity is detected with NADH or NADPH Archaeoglobus fulgidus ?
-
?
1.11.1.21 additional information the catalase activity strongly exceeds the peroxidase activity Archaeoglobus fulgidus ?
-
?
1.11.1.21 o-dianisidine + H2O2
-
Archaeoglobus fulgidus oxidized o-dianisidine + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 homodimer 2 * 85000, SDS-PAGE Archaeoglobus fulgidus
1.11.1.21 homodimer 2 * 84900, calculated from amino acid sequence Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.21 70
-
catalase activity Archaeoglobus fulgidus
1.11.1.21 80
-
peroxidase activity Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 4.5
-
peroxidase activity Archaeoglobus fulgidus
1.11.1.21 6
-
catalase activity Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme the enzyme possesses 0.25 heme per subunit Archaeoglobus fulgidus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.02
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus hydroxylamine
1.11.1.21 0.025
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus azide
1.11.1.21 0.15
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus azide
1.11.1.21 1
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus cyanide
1.11.1.21 1
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus cyanide
1.11.1.21 1.5
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus hydroxylamine
1.11.1.21 6
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus 3-Amino-1,2,4-triazole
1.11.1.21 30
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus 3-Amino-1,2,4-triazole