BRENDA - Enzyme Database show

Characterization of a catalase-peroxidase from the hyperthermophilic archaeon Archaeoglobus fulgidus

Kengen, S.W.; Bikker, F.J.; Hagen, W.R.; de Vos, W.M.; van der Oost, J.; Extremophiles 5, 323-332 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.11.1.21
expressed in Escherichia coli BL21(DE3) cells
Archaeoglobus fulgidus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.21
3-Amino-1,2,4-triazole
-
Archaeoglobus fulgidus
1.11.1.21
azide
inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
1.11.1.21
cyanide
inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
1.11.1.21
H2O2
at a H2O2 concentration of 1 mM, a half-life of inactivation of approximately 60 s is observed, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) protects the enzyme from inactivation by H2O2
Archaeoglobus fulgidus
1.11.1.21
hydroxylamine
; inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.21
0.85
-
H2O2
peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.21
84900
-
2 * 84900, calculated from amino acid sequence
Archaeoglobus fulgidus
1.11.1.21
85000
-
2 * 85000, SDS-PAGE
Archaeoglobus fulgidus
1.11.1.21
169000
-
gel filtration
Archaeoglobus fulgidus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.21
Archaeoglobus fulgidus
O28050
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.21
Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.21
0.45
-
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
1.11.1.21
23.3
-
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
1.11.1.21
2375
-
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
Archaeoglobus fulgidus
1.11.1.21
6922
-
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
Archaeoglobus fulgidus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.21
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
-
-
?
1.11.1.21
2,4-dichlorophenol + H2O2
-
714935
Archaeoglobus fulgidus
?
-
-
-
?
1.11.1.21
3,3'-diaminobenzidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 3,3'-diaminobenzidine + H2O
-
-
-
?
1.11.1.21
3,3'-dimethoxybenzidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 3,3'-dimethoxybenzidine + H2O
-
-
-
?
1.11.1.21
4-phenylenediamine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 4-phenylenediamine + H2O
-
-
-
?
1.11.1.21
aminoantipyrine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized aminoantipyrine + H2O
-
-
-
?
1.11.1.21
H2O2
-
714935
Archaeoglobus fulgidus
O2 + H2O
-
-
-
?
1.11.1.21
additional information
no activity is detected with NADH or NADPH
714935
Archaeoglobus fulgidus
?
-
-
-
-
1.11.1.21
additional information
the catalase activity strongly exceeds the peroxidase activity
714935
Archaeoglobus fulgidus
?
-
-
-
-
1.11.1.21
o-dianisidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized o-dianisidine + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.21
homodimer
2 * 84900, calculated from amino acid sequence; 2 * 85000, SDS-PAGE
Archaeoglobus fulgidus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.21
70
-
catalase activity
Archaeoglobus fulgidus
1.11.1.21
80
-
peroxidase activity
Archaeoglobus fulgidus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.21
4.5
-
peroxidase activity
Archaeoglobus fulgidus
1.11.1.21
6
-
catalase activity
Archaeoglobus fulgidus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.21
heme
the enzyme possesses 0.25 heme per subunit
Archaeoglobus fulgidus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.11.1.21
0.02
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
hydroxylamine
1.11.1.21
0.025
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
azide
1.11.1.21
0.15
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
azide
1.11.1.21
1
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C; peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
cyanide
1.11.1.21
1.5
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
hydroxylamine
1.11.1.21
6
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
3-Amino-1,2,4-triazole
1.11.1.21
30
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
3-Amino-1,2,4-triazole
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.21
expressed in Escherichia coli BL21(DE3) cells
Archaeoglobus fulgidus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.21
heme
the enzyme possesses 0.25 heme per subunit
Archaeoglobus fulgidus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.11.1.21
0.02
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
hydroxylamine
1.11.1.21
0.025
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
azide
1.11.1.21
0.15
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
azide
1.11.1.21
1
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C; peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
cyanide
1.11.1.21
1.5
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
hydroxylamine
1.11.1.21
6
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
3-Amino-1,2,4-triazole
1.11.1.21
30
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
3-Amino-1,2,4-triazole
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.21
3-Amino-1,2,4-triazole
-
Archaeoglobus fulgidus
1.11.1.21
azide
inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
1.11.1.21
cyanide
inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
1.11.1.21
H2O2
at a H2O2 concentration of 1 mM, a half-life of inactivation of approximately 60 s is observed, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) protects the enzyme from inactivation by H2O2
Archaeoglobus fulgidus
1.11.1.21
hydroxylamine
; inhibits the peroxidase activity of the enzyme
Archaeoglobus fulgidus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.21
0.85
-
H2O2
peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.21
84900
-
2 * 84900, calculated from amino acid sequence
Archaeoglobus fulgidus
1.11.1.21
85000
-
2 * 85000, SDS-PAGE
Archaeoglobus fulgidus
1.11.1.21
169000
-
gel filtration
Archaeoglobus fulgidus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.21
Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.21
0.45
-
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
1.11.1.21
23.3
-
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C
Archaeoglobus fulgidus
1.11.1.21
2375
-
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
Archaeoglobus fulgidus
1.11.1.21
6922
-
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.21
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
-
-
?
1.11.1.21
2,4-dichlorophenol + H2O2
-
714935
Archaeoglobus fulgidus
?
-
-
-
?
1.11.1.21
3,3'-diaminobenzidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 3,3'-diaminobenzidine + H2O
-
-
-
?
1.11.1.21
3,3'-dimethoxybenzidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 3,3'-dimethoxybenzidine + H2O
-
-
-
?
1.11.1.21
4-phenylenediamine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized 4-phenylenediamine + H2O
-
-
-
?
1.11.1.21
aminoantipyrine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized aminoantipyrine + H2O
-
-
-
?
1.11.1.21
H2O2
-
714935
Archaeoglobus fulgidus
O2 + H2O
-
-
-
?
1.11.1.21
additional information
no activity is detected with NADH or NADPH
714935
Archaeoglobus fulgidus
?
-
-
-
-
1.11.1.21
additional information
the catalase activity strongly exceeds the peroxidase activity
714935
Archaeoglobus fulgidus
?
-
-
-
-
1.11.1.21
o-dianisidine + H2O2
-
714935
Archaeoglobus fulgidus
oxidized o-dianisidine + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.21
homodimer
2 * 84900, calculated from amino acid sequence; 2 * 85000, SDS-PAGE
Archaeoglobus fulgidus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.21
70
-
catalase activity
Archaeoglobus fulgidus
1.11.1.21
80
-
peroxidase activity
Archaeoglobus fulgidus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.21
4.5
-
peroxidase activity
Archaeoglobus fulgidus
1.11.1.21
6
-
catalase activity
Archaeoglobus fulgidus