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Literature summary extracted from
- Enlightening the molecular basis of trypanothione specificity in trypanosomatids: Mutagenesis of Leishmania infantum glyoxalase II (2011), Exp. Parasitol., 129, 402-408.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.2.6 | His-tagged version of mutant protein expressed in Escherichia coli BL21-Codon plus | Leishmania infantum |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.2.6 | - |
Leishmania infantum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.2.6 | Y291R/C294K | substrate specificity changed | Leishmania infantum |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.2.6 | protein stability increased in the presence of 10 mM MnCl2 and 5% PEG (PEG4000, PEG400, PEG8000) | Leishmania infantum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.6 | 0.092 | - |
S-D-lactoyltrypanothione | wild type protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum |  |
| 3.1.2.6 | 0.15 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
| 3.1.2.6 | 0.439 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.2.6 | additional information | metalloprotein | Leishmania infantum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.6 | Leishmania infantum | Q2PYN0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.6 | immobilized metal ion affinity chromatography (Co2+), gel filtration | Leishmania infantum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.6 | S-D-lactoylglutathione + H2O | used by mutant Y291R/C294K | Leishmania infantum | D-lactate + glutathione | - |
? | |
| 3.1.2.6 | S-D-lactoyltrypanothione + H2O | additionally S-D-lactoylglutathione used by mutant Y291R/C294K | Leishmania infantum | D-lactate + trypanothione | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.6 | monomer | 295 amino acid residues | Leishmania infantum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.2.6 | glyoxylase II | - |
Leishmania infantum |
| 3.1.2.6 | hydroxyacylglutathione hydrolase | - |
Leishmania infantum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.6 | 16 | - |
S-D-lactoyltrypanothione | wild type protein, no activity observed with S-D-lactoylglutathione, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
| 3.1.2.6 | 241 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
| 3.1.2.6 | 918 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.6 | 180 | - |
S-D-lactoyltrypanothione | wild type protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
| 3.1.2.6 | 1600 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
| 3.1.2.6 | 2090 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
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