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Literature summary extracted from

  • Paletta-Silva, R.; Vieira, D.P.; Vieira-Bernardo, R.; Majerowicz, D.; Gondim, K.C.; Vannier-Santos, M.A.; Lopes, A.H.; Meyer-Fernandes, J.R.
    Leishmania amazonensis: Characterization of an ecto-3'-nucleotidase activity and its possible role in virulence (2011), Exp. Parasitol., 129, 277-283.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.6 ammonium tetrathiomolybdate 40% inhibition at 1 mM Leishmania amazonensis
3.1.3.6 Cd2+
-
 Leishmania amazonensis
3.1.3.6 Cu2+ over 95% uinhibition at 1 mM Leishmania amazonensis
3.1.3.6 molybdate
-
 Leishmania amazonensis
3.1.3.6 vanadate 30% inhibition at 1 mM Leishmania amazonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.6 additional information
-
 additional information Michaelis-Menten kinetics Leishmania amazonensis
3.1.3.6 0.088
-
 3'-AMP pH 7.4, 25°C Leishmania amazonensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.6 cell surface the 3'-nucleotidase activity is exclusively located on the Leishmania amazonensis external surface Leishmania amazonensis 9986
-
3.1.3.6 membrane
-
 Leishmania amazonensis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Leishmania amazonensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.6 promastigote
-
 Leishmania amazonensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Leishmania amazonensis adenosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.6 3'NT/NU
-
 Leishmania amazonensis
3.1.3.6 ecto-3'-nucleotidase
-
 Leishmania amazonensis
3.1.3.6 ecto-3'-nucleotidase/nuclease
-
 Leishmania amazonensis
3.1.3.6 La3'-nucleotidase
-
 Leishmania amazonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.6 25
-
 assay at Leishmania amazonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 7.4
-
 assay at Leishmania amazonensis

General Information

EC Number General Information Comment Organism
3.1.3.6 additional information ecto-3'-nucleotidase activity is 60fold higher than that of ecto-5'-nucleotidase in Leishmania amazonensis. Additionally, ecto-3'-nucleotidase activity is 2fold higher in virulent Leishmania amazonensis cells than in avirulent ones Leishmania amazonensis
3.1.3.6 physiological function La3'-nucleotidase plays a significant role in the generation of adenosine, which contribute to mammalian host immune response impairment and establishment of infection, possible involvement of ecto-3'-nucleotidase in cellular adhesion Leishmania amazonensis