Any feedback?
Literature summary extracted from
- Entamoeba histolytica: biochemical characterization of a protein disulfide isomerase (2011), Exp. Parasitol., 128, 76-81.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | medicine | PDI represents a potential drug target for anti-amebic therapy | Entamoeba histolytica |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.4.1 | expressed in Escherichia coli XL1-Blue MRF' cells | Entamoeba histolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.4.1 | bacitracin | - |
Entamoeba histolytica |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.4.1 | 0.0116 | - |
scrambled reoxidized lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
| 5.3.4.1 | 0.0253 | - |
denatured-reduced lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
| 5.3.4.1 | 2.026 | - |
Insulin | pH 7.0, temperature not specified in the publication | Entamoeba histolytica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.4.1 | Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.4.1 | Ni-NTA-agarose column chromatography, gel filtration | Entamoeba histolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.4.1 | denatured-reduced lysozyme | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| 5.3.4.1 | insulin | reductase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| 5.3.4.1 | additional information | PDI behaves mainly as an oxidase/isomerase and exhibits chaperone-like activity | Entamoeba histolytica | ? | - |
? | |
| 5.3.4.1 | RNase A | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| 5.3.4.1 | scrambled reoxidized lysozyme | isomerase activity of PDI | Entamoeba histolytica | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | PDI | - |
Entamoeba histolytica |
| 5.3.4.1 | protein disulfide isomerase | - |
Entamoeba histolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.4.1 | 6.5 | - |
pH optimum for the reductase activity of recombinant PDI | Entamoeba histolytica |
| 5.3.4.1 | 8 | - |
pH optimum for the oxidase activity of recombinant PDI | Entamoeba histolytica |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 5.3.4.1 | 0.172 | - |
using denatured-reduced lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
| 5.3.4.1 | 0.334 | - |
using scrambled reoxidized lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
| 5.3.4.1 | 1.137 | - |
using insulin as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
