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Literature summary extracted from

  • Jensen, K.K.; Geoghagen, N.S.; Jin, L.; Holt, T.G.; Luo, Q.; Malkowitz, L.; Ni, W.; Quan, S.; Waters, M.G.; Zhang, A.; Zhou, H.H.; Cheng, K.; Luo, M.J.
    Pharmacological activation and genetic manipulation of cystathionine beta-synthase alter circulating levels of homocysteine and hydrogen sulfide in mice (2011), Eur. J. Pharmacol., 650, 86-93.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 Ethionine i.e. 2-amino-4-(ethylthio)butyric acid, an methionine analogue, which is converted to S-adenosyl-ethionine in vivo and activates CBS, treatment increased liver CBS activity 4.0fold in wild-type mice Mus musculus
4.2.1.22 S-adenosyl-L-methionine
-
 Mus musculus
4.2.1.22 S-adenosyl-L-methionine
-
 Rattus norvegicus
4.2.1.22 S-adenosyl-L-methionine activates CBS by 13.6fold in Hep-G2 cell lysate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 additional information CBS activity in liver homogenates is reduced and plasma homocysteine levels are elevated in the CBS heterozygous knockout , CBS-/+ animals compared to wild-type littermate control mice. H2S is also significantly reduced by 30% and 46% compared to wild type in male and female CBS-/+ animals respectively Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.22 L-serine + L-homocysteine Mus musculus
-
 L-cystathionine + H2O
-
 ?
4.2.1.22 L-serine + L-homocysteine Homo sapiens
-
 L-cystathionine + H2O
-
 ?
4.2.1.22 L-serine + L-homocysteine Rattus norvegicus
-
 L-cystathionine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens
-
-
-
4.2.1.22 Mus musculus
-
-
-
4.2.1.22 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.22 Hep-G2 cell
-
 Homo sapiens
-
4.2.1.22 liver
-
 Mus musculus
-
4.2.1.22 liver
-
 Homo sapiens
-
4.2.1.22 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-serine + L-homocysteine
-
 Mus musculus L-cystathionine + H2O
-
 ?
4.2.1.22 L-serine + L-homocysteine
-
 Homo sapiens L-cystathionine + H2O
-
 ?
4.2.1.22 L-serine + L-homocysteine
-
 Rattus norvegicus L-cystathionine + H2O
-
 ?
4.2.1.22 additional information CBS also catalyze H2S production in vitro Mus musculus ?
-
 ?
4.2.1.22 additional information CBS also catalyze H2S production in vitro Homo sapiens ?
-
 ?
4.2.1.22 additional information CBS also catalyze H2S production in vitro Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 CBS
-
 Mus musculus
4.2.1.22 CBS
-
 Homo sapiens
4.2.1.22 CBS
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.22 37
-
 assay at Mus musculus
4.2.1.22 37
-
 assay at Homo sapiens
4.2.1.22 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.22 8.5
-
 assay at Mus musculus
4.2.1.22 8.5
-
 assay at Homo sapiens
4.2.1.22 8.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 pyridoxal 5'-phosphate
-
 Mus musculus
4.2.1.22 pyridoxal 5'-phosphate
-
 Homo sapiens
4.2.1.22 pyridoxal 5'-phosphate
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
4.2.1.22 additional information increased immunoreactivity is evident in liver homogenates from mice treated with adenovirus carrying human CBS compared to mice treated with the Ad-lacZ virus Homo sapiens
4.2.1.22 additional information increased immunoreactivity is evident in liver homogenates from mice treated with adenovirus carrying human CBS compared to mice treated with the Ad-lacZ virus. Overexpression of hCBS reduces homocysteine levels significantly by 5.3fold compared to Ad-lacZ treated mice Mus musculus
4.2.1.22 physiological function CBS activity partially regulates endogenous H2S in mice. It contributes significantly to endogenous H2S production in mice, adenovirus-mediated overexpression of CBS in the liver significantly increases circulating levels of H2S, whereas CBS deficiency results in reduced levels. irculating H2S levels are increased by pharmacological activation of CBS in vivo; i.e. in the presence of the endogenous activator Mus musculus