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Literature summary extracted from

  • Flores-Maltos, A.; Rodriguez-Duran, L.V.; Renovato, J.; Contreras, J.C.; Rodriguez, R.; Aguilar, C.N.
    Catalytical properties of free and immobilized Aspergillus niger tannase (2011), Enzyme Res., 2011, 768183.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.4
-
 Tannic acid free enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C Aspergillus niger
3.1.1.20 0.433
-
 methyl gallate free enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C Aspergillus niger
3.1.1.20 0.529
-
 methyl gallate Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C Aspergillus niger
3.1.1.20 23.75
-
 Tannic acid Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
-
-
3.1.1.20 Aspergillus niger GH1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 partial purification by HiTrap G25 gel filtration (5.4fold purification and 5.6fold concentration with a recovery yield higher than 90%) Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Aspergillus niger GH1 gallate + methanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger gallic acid + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger GH1 gallic acid + D-glucose
-
 ?