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Literature summary extracted from

  • Kourtoglou, E.; Anasontzis, G.; Mamma, D.; Topakas, E.; Hatzinikolaou, D.; Christakopoulos, P.
    Constitutive expression, purification and characterization of a phosphoglucomutase from Fusarium oxysporum (2011), Enzyme Microb. Technol., 48, 217-224.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.4.2.2 loss of activity is observed when phosphate buffer is used in the pH range of 6.0-8.0 Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.00103
-
 D-glucose 1,6-diphosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum
5.4.2.2 0.1
-
 alpha-D-glucose 1-phosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Ca2+ the enzyme is also active with 5 mM Ca2+ but to a lesser extent as Co2+ (11.9% relative activity) Fusarium oxysporum
5.4.2.2 Co2+ PGM requires the presence of a divalent cation for its function with maximum activity (100%) being obtained with 5 mM Co2+ Fusarium oxysporum
5.4.2.2 Mg2+ the enzyme is also active with 5 mM Mg2+ but to a lesser extent as Co2+ (24.5% relative activity) Fusarium oxysporum
5.4.2.2 Mn2+ the enzyme is also active with 5 mM Mn2+ but to a lesser extent as Co2+ (69.5% relative activity) Fusarium oxysporum
5.4.2.2 Ni2+ the enzyme is also active with 5 mM Ni2+ but to a lesser extent as Co2+ (22.5% relative activity) Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 56000
-
 1 * 56000, SDS-PAGE Fusarium oxysporum
5.4.2.2 60000
-
 gel filtration Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Fusarium oxysporum
-
-
-
5.4.2.2 Fusarium oxysporum F3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 Q-Sepharose column chromatography and Sephacryl-S 100 gel filtration Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 1.1
-
 crude enzyme, at pH 7.6, 25°C Fusarium oxysporum
5.4.2.2 5.3
-
 after 5fold purification, at pH 7.6, 25°C Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Fusarium oxysporum alpha-D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Fusarium oxysporum F3 alpha-D-glucose 6-phosphate
-
 ?
5.4.2.2 D-glucose 1,6-diphosphate
-
 Fusarium oxysporum ?
-
 ?
5.4.2.2 D-glucose 1,6-diphosphate
-
 Fusarium oxysporum F3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 monomer 1 * 56000, SDS-PAGE Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PGM
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.2 45
-
-
 Fusarium oxysporum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.2.2 15 50
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.2 30 60 PGM is almost stable (12% loss of the activity) at 30°C for 60 min, while almost complete loss of activity is observed at 50 and 60°C after 10 min Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.2 0.74
-
 alpha-D-glucose 1-phosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum
5.4.2.2 0.74
-
 D-glucose 1,6-diphosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.2 7
-
 using 3-(N-morpholino)propanesulfonic acid buffer Fusarium oxysporum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.2.2 6 8 using 3-(N-morpholino)propanesulfonic acid buffer, the enzyme retains up to 90% of its initial activity Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.2.2 7 10 at pH 7.0 only 17.5% of the relative activity is recovered, the enzyme retains 14% of its initial activity after incubation at pH 10.0 Fusarium oxysporum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.4.2.2 Fusarium oxysporum isoelectric focusing
-
 3.5

General Information

EC Number General Information Comment Organism
5.4.2.2 metabolism PGM is a key enzyme in carbohydrate metabolism, where it catalyzes the reversible transfer of a phosphate group between C-1 and C-6 of D-glucose via D-glucose 1,6-diphosphate Fusarium oxysporum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.2.2 7.38
-
 alpha-D-glucose 1-phosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum
5.4.2.2 714.3
-
 D-glucose 1,6-diphosphate in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C Fusarium oxysporum