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Literature summary extracted from

  • Giglio, S.; Chou, W.K.; Ikeda, H.; Cane, D.E.; Monis, P.T.
    Biosynthesis of 2-methylisoborneol in cyanobacteria (2010), Environ. Sci. Technol., 45, 992-998.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.255 DNA and amino acid sequence determination and analysis, expression in Escherichia coli Pseudanabaena limnetica
2.1.1.255 expression in Escherichia coli as N-terminal His6-tagged protein Pseudanabaena limnetica
2.1.1.255 expression in Escherichia coli as N-terminal His6-tagged protein Oscillatoria limosa
2.1.1.255 expression in Escherichia coli as N-terminal His6-tagged protein Pseudanabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena limnetica
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Oscillatoria limosa
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena sp.
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena limnetica Castaic Lake
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena sp. NIVA-CYA 111
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Oscillatoria limosa LBD 305b
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.255 Oscillatoria limosa E9NH31
-
-
2.1.1.255 Oscillatoria limosa LBD 305b E9NH31
-
-
2.1.1.255 Pseudanabaena limnetica E9NH27
-
-
2.1.1.255 Pseudanabaena limnetica E9NH27 isolated from Castaic Lake
-
2.1.1.255 Pseudanabaena limnetica Castaic Lake E9NH27
-
-
2.1.1.255 Pseudanabaena sp. E9NH33
-
-
2.1.1.255 Pseudanabaena sp. NIVA-CYA 111 E9NH33
-
-
4.2.3.118 Pseudanabaena limnetica E9NH28
-
-
4.2.3.118 Pseudanabaena limnetica Castaic Lake E9NH28
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.255
-
 Pseudanabaena limnetica
2.1.1.255
-
 Oscillatoria limosa
2.1.1.255
-
 Pseudanabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena limnetica S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Oscillatoria limosa S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena sp. S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena limnetica Castaic Lake S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena sp. NIVA-CYA 111 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Oscillatoria limosa LBD 305b S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O
-
 Pseudanabaena limnetica 2-methylisoborneol + diphosphate major reaction product ?
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O
-
 Pseudanabaena limnetica Castaic Lake 2-methylisoborneol + diphosphate major reaction product ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.255 2-methyl-GPP synthase
-
 Pseudanabaena limnetica
2.1.1.255 2-methyl-GPP synthase
-
 Oscillatoria limosa
2.1.1.255 2-methyl-GPP synthase
-
 Pseudanabaena sp.
2.1.1.255 geranyl diphosphate 2-methyltransferase
-
 Pseudanabaena limnetica
2.1.1.255 GPP 2-methyl transferase
-
 Pseudanabaena limnetica
2.1.1.255 GPP methyltransferase
-
 Pseudanabaena limnetica
2.1.1.255 GPP methyltransferase
-
 Oscillatoria limosa
2.1.1.255 GPP methyltransferase
-
 Pseudanabaena sp.
2.1.1.255 GPPMT
-
 Pseudanabaena limnetica
2.1.1.255 GPPMT
-
 Oscillatoria limosa
2.1.1.255 GPPMT
-
 Pseudanabaena sp.
2.1.1.255 MGPPS
-
 Pseudanabaena limnetica
2.1.1.255 MGPPS
-
 Oscillatoria limosa
2.1.1.255 MGPPS
-
 Pseudanabaena sp.
4.2.3.118 MIBS
-
 Pseudanabaena limnetica

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.255 S-adenosyl-L-methionine
-
 Pseudanabaena limnetica

General Information

EC Number General Information Comment Organism
2.1.1.255 metabolism the enzyme catalyzes the first step in the biosynthesis of 2-methylisoborneol, overview Pseudanabaena limnetica
4.2.3.118 physiological function biosynthesis of 2-methylisoborneol in cyanobacteria is a result of 2 key reactions, a S-adenosylmethionine-dependent methylation of the monoterpene precursor geranyl diphosphate to 2-methylgeranyl diphosphate catalyzed by geranyl diphosphate 2-methyltransferase, and further cyclization of 2-methylgeranyl diphosphate to 2-methylisoborneol catalyzed by 2-methylisoborneol synthase as part of a 2-methylisoborneol operon Pseudanabaena limnetica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.118 1.45
-
 (E)-2-methylgeranyl diphosphate temperature not specified in the publication, pH not specified in the publication Pseudanabaena limnetica