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Literature summary extracted from

  • Huang, H.; Zhang, R.; Fu, D.; Luo, J.; Li, Z.; Luo, H.; Shi, P.; Yang, P.; Diao, Q.; Yao, B.
    Diversity, abundance and characterization of ruminal cysteine phytases suggest their important role in phytate degradation (2011), Environ. Microbiol., 13, 747-757.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis Bos taurus
3.1.3.8 genes phyG1 and phyG51, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis Capra hircus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular secreted phytases PhyG1 and PhyG51 Capra hircus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Bos taurus
-
-
-
3.1.3.8 Capra hircus
-
 genes phyG1 and phyG51
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 cysteine phytase
-
 Bos taurus
3.1.3.8 cysteine phytase
-
 Capra hircus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
-
 Bos taurus
3.1.3.8 50
-
-
 Capra hircus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 37 50 80% of maximal activity at 39°C, maximal activity at 50°C Bos taurus
3.1.3.8 37 50 80% of maximal activity at 39°C, maximal activity at 50°C Capra hircus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 80
-
 isozyme r-PhyG1 loses over 80% of its activity after incubation at 50°C for 15 min, whereas isozyme r-phyG51 is not affected by these conditions Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6
-
 secreted isozyme PhyG1 Capra hircus
3.1.3.8 6.5
-
 secreted isozyme PhyG51 Capra hircus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 5 7 the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 Bos taurus
3.1.3.8 5 7 the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 Capra hircus

General Information

EC Number General Information Comment Organism
3.1.3.8 physiological function cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment Bos taurus
3.1.3.8 physiological function cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment Capra hircus