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Literature summary extracted from

  • Gavina, J.M.; White, C.E.; Finan, T.M.; Britz-McKibbin, P.
    Determination of 4-hydroxyproline-2-epimerase activity by capillary electrophoresis: A stereoselective platform for inhibitor screening of amino acid isomerases (2010), Electrophoresis, 31, 2831-2837.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.8 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.8 additional information not inhibited by 5 mM trans-3-hydroxy-L-proline, P5C, and L-proline Pseudomonas aeruginosa
5.1.1.8 pyrrole 2-carboxylic acid selective inhibitor, competitive inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.8 0.0016
-
 trans-4-hydroxy-D-proline in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa
5.1.1.8 0.0024
-
 trans-4-hydroxy-L-proline in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.8 cobalt resin column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.8 trans-4-hydroxy-D-proline
-
 Pseudomonas aeruginosa cis-4-hydroxy-L-proline
-
 r
5.1.1.8 trans-4-hydroxy-L-proline
-
 Pseudomonas aeruginosa cis-4-hydroxy-D-proline
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.8 4-hydroxyproline-2-epimerase
-
 Pseudomonas aeruginosa
5.1.1.8 HyPRE
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.8 additional information the enzyme is pyridoxal 5'-phosphate-independent Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.1.1.8 2.3
-
 in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C Pseudomonas aeruginosa pyrrole 2-carboxylic acid