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Literature summary extracted from

  • Padhi, S.K.; Fujii, R.; Legatt, G.A.; Fossum, S.L.; Berchtold, R.; Kazlauskas, R.J.
    Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions (2010), Chem. Biol., 17, 863-871.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 1.2
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Hevea brasiliensis
4.1.2.47 1.4
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Hevea brasiliensis
-
-
-
4.1.2.47 Manihot esculenta
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 (S)-mandelonitrile
-
 Hevea brasiliensis cyanide + benzaldehyde
-
 ?
4.1.2.47 (S)-mandelonitrile
-
 Manihot esculenta cyanide + benzaldehyde
-
 r
4.1.2.47 cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile low enantioselectivity with 20% ee (S) r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-selective hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 HNL
-
 Hevea brasiliensis
4.1.2.47 HNL
-
 Manihot esculenta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.47 1.83
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Hevea brasiliensis
4.1.2.47 21.67
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Manihot esculenta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.47 1.55
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Hevea brasiliensis
4.1.2.47 15.83
-
 (S)-mandelonitrile in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication Manihot esculenta