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Literature summary extracted from

  • Yang, W.; Xue, L.; Fang, L.; Chen, X.; Zhan, C.
    Characterization of a high-activity mutant of human butyrylcholinesterase against (-)-cocaine (2010), Chem. Biol. Interact., 187, 148-152.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.84 A199S/S287G/A328W/Y332G the mutant shows high activity towards (-)-cocaine with about 1080fold (un-fused) and 100fold (when fused with human serum albumin) improved catalytic efficiency compared to the wild type enzyme and also leads to a decrease in catalytic efficiency with acetylthiocholine and butyrylthiocholine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.0083
-
 butyrylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 0.021
-
 butyrylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 0.029
-
 acetylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 0.037
-
 acetylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 0.0031
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 0.0045
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-
3.1.1.84 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetylthiocholine + H2O
-
 Homo sapiens thiocholine + acetate
-
 ?
3.1.1.8 butyrylthiocholine + H2O best enzyme for the wild type enzyme Homo sapiens thiocholine + butyrate
-
 ?
3.1.1.84 (-)-cocaine + H2O
-
 Homo sapiens benzoic acid + methyl-(1R,2R,3S,5S)-3-hydroxy-8-methyl-8-azabicyclo[3.2.1]-octane-2-carboxylate i.e. ecgonine methyl ester ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 BChE
-
 Homo sapiens
3.1.1.8 butyrylcholinesterase
-
 Homo sapiens
3.1.1.84 BChE
-
 Homo sapiens
3.1.1.84 butyrylcholinesterase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 46.67
-
 butyrylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 160.2
-
 acetylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 336.7
-
 acetylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 491.7
-
 butyrylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 0.068
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 51
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.8 4333
-
 acetylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 11500
-
 acetylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 23330
-
 butyrylthiocholine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.8 56670
-
 butyrylthiocholine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 15.17
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
3.1.1.84 16500
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens