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Literature summary extracted from

  • Matsui, D.; Oikawa, T.
    Detection and function of the intramolecular disulfide bond in arginine racemase: an enzyme with broad substrate specificity (2010), Chem. Biodivers., 7, 1591-1602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.9 expressed in Escherichia coli BL21(DE3) cells Pseudomonas taetrolens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.1.9 C47A/C73A the mutant displays about 7fold reduced specific activity compared to the wild type enzyme. The relative activities of C47A/C73AArgR for L-arginine, L-lysine, L-ornithine, D-arginine, D-lysine, and D-ornithine correspond well to those of wild type ArgR, but the relative activities for L-alanine and D-alanine increase to 25 and 26%, respectively Pseudomonas taetrolens

General Stability

EC Number General Stability Organism
5.1.1.9 the disulfide bond of ArgR is essential for ArgR to fold Pseudomonas taetrolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.9 1
-
 L-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 1
-
 D-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 1.2
-
 L-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 1.2
-
 D-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 1.8
-
 L-lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 1.8
-
 D-Lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 2.4
-
 L-lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 2.4
-
 D-Lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 3
-
 L-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 3.1
-
 D-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 3.6
-
 D-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 3.7
-
 L-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 8.4
-
 L-alanine mutant enzyme C74A/C73A ,in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 8.8
-
 D-alanine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 15
-
 D-alanine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 15.7
-
 L-alanine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.9 cytoplasm an about 30times higher activity of ArgR is detected in the periplasm compared to that in the cytoplasm Pseudomonas taetrolens 5737
-
5.1.1.9 periplasm an about 30times higher activity of ArgR is detected in the periplasm compared to that in the cytoplasm Pseudomonas taetrolens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.9 42000
-
 2 * 42000 Pseudomonas taetrolens

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.9 Pseudomonas taetrolens
-
-
-
5.1.1.9 Pseudomonas taetrolens NBRC 3460
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.9
-
 Pseudomonas taetrolens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.9 10.5
-
 periplasmic arginine racemase activity, mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 70.2
-
 periplasmic arginine racemase activity, wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.9 D-alanine 11% activity compared to D-arginine Pseudomonas taetrolens L-alanine
-
 ?
5.1.1.9 D-alanine 11% activity compared to D-arginine Pseudomonas taetrolens NBRC 3460 L-alanine
-
 ?
5.1.1.9 D-arginine 100% activity Pseudomonas taetrolens L-arginine
-
 ?
5.1.1.9 D-arginine 100% activity Pseudomonas taetrolens NBRC 3460 L-arginine
-
 ?
5.1.1.9 D-lysine 103% activity compared to D-arginine Pseudomonas taetrolens L-lysine
-
 ?
5.1.1.9 D-ornithine 42% activity compared to D-arginine Pseudomonas taetrolens L-ornithine
-
 ?
5.1.1.9 L-alanine 12% activity compared to L-arginine Pseudomonas taetrolens D-alanine
-
 ?
5.1.1.9 L-alanine 12% activity compared to L-arginine Pseudomonas taetrolens NBRC 3460 D-alanine
-
 ?
5.1.1.9 L-arginine 100% activity Pseudomonas taetrolens D-arginine
-
 ?
5.1.1.9 L-arginine 100% activity Pseudomonas taetrolens NBRC 3460 D-arginine
-
 ?
5.1.1.9 L-lysine 104% activity compared to L-arginine Pseudomonas taetrolens D-lysine
-
 ?
5.1.1.9 L-Ornithine 44% activity compared to L-arginine Pseudomonas taetrolens D-Ornithine
-
 ?
5.1.1.9 L-Ornithine 44% activity compared to L-arginine Pseudomonas taetrolens NBRC 3460 D-Ornithine
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.9 homodimer 2 * 42000 Pseudomonas taetrolens

Synonyms

EC Number Synonyms Comment Organism
5.1.1.9 ArgR
-
 Pseudomonas taetrolens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.9 2.4
-
 L-alanine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 2.6
-
 D-alanine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 6.3
-
 L-alanine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 6.8
-
 D-alanine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 67.5
-
 D-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 68.8
-
 L-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 81.7
-
 D-Lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 83.3
-
 D-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 84.9
-
 L-lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 87.7
-
 L-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 93.5
-
 L-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 94.6
-
 D-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 104
-
 D-Lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 109
-
 L-lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 119
-
 L-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 119
-
 D-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.9 pyridoxal 5'-phosphate dependent on Pseudomonas taetrolens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.1.9 0.15
-
 L-alanine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 0.17
-
 D-alanine wild type enzyme,in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 0.74
-
 L-alanine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 0.77
-
 D-alanine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 22.1
-
 D-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 22.9
-
 L-ornithine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 23
-
 D-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 23.4
-
 L-ornithine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 43.7
-
 D-Lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 45.1
-
 D-Lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 46.2
-
 L-lysine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 47.4
-
 L-lysine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 92.6
-
 L-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 94.6
-
 D-arginine mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 96
-
 D-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens
5.1.1.9 101
-
 L-arginine wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C Pseudomonas taetrolens