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Literature summary extracted from

  • Okada, C.; Nakamura, A.; Tomioka, S.; Kohama, K.; Kaneko, T.S.
    Purification and characterization of the plasmodial phosphatase that hydrolyses the phosphorylated light chain of Physarum myosin II from Physarum polycephalum (2010), Cell Biol. Int., 34, 827-835.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.53 Ca2+ inhibits at 0.01 mM Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.53 0.01
-
 [myosin light-chain] phosphate pH 7.6, temperature not specified in the publication Physarum polycephalum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.53 Mg2+ activates at 0.0085 mM Physarum polycephalum
3.1.3.53 Mn2+ highly activating at 0.0016 mM Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.53 50000
-
 gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.53 [myosin light-chain] phosphate + H2O Physarum polycephalum
-
 [myosin light-chain] + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.53 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.53 native enzyme 340fold by ion exchange and hydrophobic interaction chromatography Physarum polycephalum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.53 0.028
-
 purified enzyme, pH 7.6, temperature not specified in the publication Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.53 [myosin light-chain] phosphate + H2O
-
 Physarum polycephalum [myosin light-chain] + phosphate
-
 ?
3.1.3.53 [myosin light-chain] phosphate + H2O substrate is phosphorylated Physarum myosin II Physarum polycephalum [myosin light-chain] + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.53 plasmodial phosphatase
-
 Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.53 7.6
-
 assay at Physarum polycephalum