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Literature summary extracted from
- Comparative study of two purified inulinases from thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 grown on Cichorium intybus l (2011), Braz. J. Microbiol., 42, 633-649.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.18 | additional information | - |
additional information | KM-value for inulin is 2.15 mg/ml | Thermothielavioides terrestris | |
| 4.2.2.18 | additional information | - |
additional information | KM-value for inulin is 3.45 mg/ml | Aspergillus foetidus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.2.18 | soluble | - |
Aspergillus foetidus | - |
- |
| 4.2.2.18 | soluble | - |
Thermothielavioides terrestris | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 72000 | - |
x * 72000, SDS-PAGE | Thermothielavioides terrestris |
| 4.2.2.18 | 78000 | - |
x * 78000, SDS-PAGE | Aspergillus foetidus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.18 | Aspergillus foetidus | - |
growth on 4% Cichorium intybus root extract | - |
| 4.2.2.18 | Aspergillus foetidus NRRL 337 | - |
growth on 4% Cichorium intybus root extract | - |
| 4.2.2.18 | Thermothielavioides terrestris | - |
growth on 4% Cichorium intybus root extract | - |
| 4.2.2.18 | Thermothielavioides terrestris NRRL 8126 | - |
growth on 4% Cichorium intybus root extract | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.18 | - |
Aspergillus foetidus |
| 4.2.2.18 | - |
Thermothielavioides terrestris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 100.9 | - |
pH 6.0, 440°C | Aspergillus foetidus |
| 4.2.2.18 | 254.9 | - |
pH 6.0, 440°C | Thermothielavioides terrestris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.18 | inulin | - |
Aspergillus foetidus | di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? |  |
| 4.2.2.18 | inulin | - |
Thermothielavioides terrestris | di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? | |
| 4.2.2.18 | inulin | - |
Aspergillus foetidus NRRL 337 | di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? | |
| 4.2.2.18 | inulin | - |
Thermothielavioides terrestris NRRL 8126 | di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.18 | ? | x * 78000, SDS-PAGE | Aspergillus foetidus |
| 4.2.2.18 | ? | x * 72000, SDS-PAGE | Thermothielavioides terrestris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 50 | - |
- |
Aspergillus foetidus |
| 4.2.2.18 | 75 | - |
- |
Thermothielavioides terrestris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 4.5 | - |
- |
Thermothielavioides terrestris |
| 4.2.2.18 | 5.5 | - |
- |
Aspergillus foetidus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 4.2.2.18 | Aspergillus foetidus | highest production levels of inulinase at 50°C, growth on yeast extract and peptone as nitrogen sources | up |
| 4.2.2.18 | Thermothielavioides terrestris | highest production levels of inulinase at 50°C, growth on yeast extract and peptone as nitrogen sources | up |
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