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Literature summary extracted from

  • Horspool, D.R.; Coope, R.J.; Holt, R.A.
    Efficient assembly of very short oligonucleotides using T4 DNA ligase (2010), BMC Res. Notes, 3, 291.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Tequatrovirus T4
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 additional information ligation by T4 DNA ligase is dependent on the formation of a double stranded DNA duplex of at least five base pairs surrounding the site of ligation. However, ligations can be performed effectively with overhangs smaller than five base pairs and oligonucleotides as small as octamers, in the presence of a second, complementary oligonucleotide Tequatrovirus T4 ?
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Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 T4 DNA ligase
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Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
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Tequatrovirus T4