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Literature summary extracted from

  • Im, H.; Sharpe, M.L.; Strych, U.; Davlieva, M.; Krause, K.L.
    The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design (2011), BMC Microbiol., 11, 116.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.1.1.1 medicine since it is absent in humans, this enzyme is an attractive target for the development of drugs against Streptococcus pneumoniae and other bacterial pathogens Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 expressed in Escherichia coli BL21(DE3) pLysS cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.1 sitting drop vapor diffusion method, at 4°C in 1.2 M sodium citrate, 0.1 M MES, pH 7.2, and 10% (v/v) glycerol Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Streptococcus pneumoniae P0A2W8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1 ammonium sulfate precipitation, anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-alanine
-
 Streptococcus pneumoniae D-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 homodimer x-ray crystallography Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
5.1.1.1 ALR
-
 Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate
-
 Streptococcus pneumoniae