BRENDA - Enzyme Database

Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases

Leslie, N.R.; McLennan, A.G.; Safrany, S.T.; BMC Biochem. 3, 20 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.60
;
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.60
S39N
a polymorphism of hAps1 leads to the point mutation S39N
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.60
fluoride
non-competitive inhibition; non-competitive inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.60
0.011
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.013
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.019
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
0.037
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2; pH 7.6, 37°C, hApsI-39N
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.60
cytoplasm
;
Homo sapiens
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.60
Co2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
Cu2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
Mn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
additional information
Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme; Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme
Homo sapiens
3.6.1.60
Zn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.60
18500
-
x * 18500, calculation from sequence
Homo sapiens
3.6.1.60
18559
-
x * 18559, calculation from sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-tetraphosphate + AMP
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-pentaphosphate + AMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.60
Homo sapiens
Q8NFP7
-
-
3.6.1.60
Homo sapiens
Q96G61
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.60
;
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP
(2)
Homo sapiens
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP
(1)
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.60
brain
; high activity
Homo sapiens
-
3.6.1.60
GI-102 cell
-
Homo sapiens
-
3.6.1.60
heart
; low activity
Homo sapiens
-
3.6.1.60
kidney
-
Homo sapiens
-
3.6.1.60
liver
-
Homo sapiens
-
3.6.1.60
lung
; low activity
Homo sapiens
-
3.6.1.60
additional information
no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells; no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103
Homo sapiens
-
3.6.1.60
ovary
; low activity
Homo sapiens
-
3.6.1.60
pancreas
; high activity
Homo sapiens
-
3.6.1.60
PC-3 cell
;
Homo sapiens
-
3.6.1.60
placenta
-
Homo sapiens
-
3.6.1.60
prostate
; low activity
Homo sapiens
-
3.6.1.60
small intestine
low activity
Homo sapiens
-
3.6.1.60
spleen
-
Homo sapiens
-
3.6.1.60
testis
; high activity
Homo sapiens
-
3.6.1.60
thymus
low activity
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.60
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
weak activity
714610
Homo sapiens
ATP + AMP
-
-
-
?
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.60
?
x * 18500, calculation from sequence; x * 18559, calculation from sequence
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.60
0.16
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.17
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.2
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
0.4
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI; pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.60
8.5
9
-
Homo sapiens
3.6.1.60
8.5
-
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.60
7.5
9
pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity; pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.60
0.013
-
fluoride
pH 7.6, 37°C; pH 7.6, 37°C
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.60
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.60
S39N
a polymorphism of hAps1 leads to the point mutation S39N
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.60
fluoride
non-competitive inhibition
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.60
0.013
-
fluoride
pH 7.6, 37°C
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.60
0.011
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.013
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.019
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
0.037
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.60
cytoplasm
-
Homo sapiens
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.60
Co2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
Cu2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
Mn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
3.6.1.60
additional information
Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme
Homo sapiens
3.6.1.60
Zn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.60
18500
-
x * 18500, calculation from sequence
Homo sapiens
3.6.1.60
18559
-
x * 18559, calculation from sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-tetraphosphate + AMP
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-pentaphosphate + AMP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.60
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.60
brain
high activity
Homo sapiens
-
3.6.1.60
brain
-
Homo sapiens
-
3.6.1.60
GI-102 cell
-
Homo sapiens
-
3.6.1.60
heart
low activity
Homo sapiens
-
3.6.1.60
heart
-
Homo sapiens
-
3.6.1.60
kidney
-
Homo sapiens
-
3.6.1.60
liver
-
Homo sapiens
-
3.6.1.60
lung
low activity
Homo sapiens
-
3.6.1.60
lung
-
Homo sapiens
-
3.6.1.60
additional information
no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells
Homo sapiens
-
3.6.1.60
additional information
no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103
Homo sapiens
-
3.6.1.60
ovary
low activity
Homo sapiens
-
3.6.1.60
ovary
-
Homo sapiens
-
3.6.1.60
pancreas
high activity
Homo sapiens
-
3.6.1.60
pancreas
-
Homo sapiens
-
3.6.1.60
PC-3 cell
-
Homo sapiens
-
3.6.1.60
placenta
-
Homo sapiens
-
3.6.1.60
prostate
low activity
Homo sapiens
-
3.6.1.60
prostate
-
Homo sapiens
-
3.6.1.60
small intestine
low activity
Homo sapiens
-
3.6.1.60
spleen
-
Homo sapiens
-
3.6.1.60
testis
high activity
Homo sapiens
-
3.6.1.60
testis
-
Homo sapiens
-
3.6.1.60
thymus
low activity
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.60
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
weak activity
714610
Homo sapiens
ATP + AMP
-
-
-
?
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
3.6.1.60
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
3.6.1.60
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.60
?
x * 18559, calculation from sequence
Homo sapiens
3.6.1.60
?
x * 18500, calculation from sequence
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.60
0.16
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.17
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
0.2
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
0.4
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI; pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
0.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.60
8.5
9
-
Homo sapiens
3.6.1.60
8.5
-
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.60
7.5
9
pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity
Homo sapiens
3.6.1.60
7.5
9
pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.60
8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
10.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
10.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
13.1
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
14.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
16
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.60
8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
10.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
3.6.1.60
10.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
13.1
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
3.6.1.60
14.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
3.6.1.60
16
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens