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Literature summary extracted from

  • Leslie, N.R.; McLennan, A.G.; Safrany, S.T.
    Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases (2002), BMC Biochem., 3, 20.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.60
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.60 S39N a polymorphism of hAps1 leads to the point mutation S39N Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.60 fluoride non-competitive inhibition; non-competitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.60 0.011
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens
3.6.1.60 0.013
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 0.019
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hAps2 Homo sapiens
3.6.1.60 0.037
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 0.05
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hAps2 Homo sapiens
3.6.1.60 0.05
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.60 cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.60 Co2+ a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity Homo sapiens
3.6.1.60 Cu2+ a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity Homo sapiens
3.6.1.60 Mn2+ a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity Homo sapiens
3.6.1.60 additional information Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme Homo sapiens
3.6.1.60 Zn2+ a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.60 18500
-
 x * 18500, calculation from sequence Homo sapiens
3.6.1.60 18559
-
 x * 18559, calculation from sequence Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O Homo sapiens marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels adenosine 5'-tetraphosphate + AMP
-
 ?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Homo sapiens marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels adenosine 5'-pentaphosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.60 Homo sapiens Q8NFP7
-
-
3.6.1.60 Homo sapiens Q96G61
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.60
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP (2) Homo sapiens
a
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP (1) Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.60 brain
-
 Homo sapiens
-
3.6.1.60 brain high activity Homo sapiens
-
3.6.1.60 GI-102 cell
-
 Homo sapiens
-
3.6.1.60 heart
-
 Homo sapiens
-
3.6.1.60 heart low activity Homo sapiens
-
3.6.1.60 kidney
-
 Homo sapiens
-
3.6.1.60 liver
-
 Homo sapiens
-
3.6.1.60 lung
-
 Homo sapiens
-
3.6.1.60 lung low activity Homo sapiens
-
3.6.1.60 additional information no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells Homo sapiens
-
3.6.1.60 additional information no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103 Homo sapiens
-
3.6.1.60 ovary
-
 Homo sapiens
-
3.6.1.60 ovary low activity Homo sapiens
-
3.6.1.60 pancreas
-
 Homo sapiens
-
3.6.1.60 pancreas high activity Homo sapiens
-
3.6.1.60 PC-3 cell
-
 Homo sapiens
-
3.6.1.60 placenta
-
 Homo sapiens
-
3.6.1.60 prostate
-
 Homo sapiens
-
3.6.1.60 prostate low activity Homo sapiens
-
3.6.1.60 small intestine low activity Homo sapiens
-
3.6.1.60 spleen
-
 Homo sapiens
-
3.6.1.60 testis
-
 Homo sapiens
-
3.6.1.60 testis high activity Homo sapiens
-
3.6.1.60 thymus low activity Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.60 P1,P4-bis(5'-adenosyl)tetraphosphate + H2O weak activity Homo sapiens ATP + AMP
-
 ?
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels Homo sapiens adenosine 5'-tetraphosphate + AMP
-
 ?
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity Homo sapiens adenosine 5'-tetraphosphate + AMP
-
 ?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels Homo sapiens adenosine 5'-pentaphosphate + AMP
-
 ?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity Homo sapiens adenosine 5'-pentaphosphate + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.60 ? x * 18500, calculation from sequence Homo sapiens
3.6.1.60 ? x * 18559, calculation from sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.60 hAps1
-
 Homo sapiens
3.6.1.60 hAps2
-
 Homo sapiens
3.6.1.60 NUDT10
-
 Homo sapiens
3.6.1.60 NUDT11
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.60 0.16
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens
3.6.1.60 0.17
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 0.2
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hAps2 Homo sapiens
3.6.1.60 0.4
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 0.4
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens
3.6.1.60 0.8
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hAps2 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.60 8.5
-
-
 Homo sapiens
3.6.1.60 8.5 9
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.60 7.5 9 pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity Homo sapiens
3.6.1.60 7.5 9 pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.60 0.013
-
 fluoride pH 7.6, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.60 8
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens
3.6.1.60 10.5
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hAps2 Homo sapiens
3.6.1.60 10.8
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 13.1
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hApsI Homo sapiens
3.6.1.60 14.5
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C, hApsI-39N Homo sapiens
3.6.1.60 16
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 7.6, 37°C, hAps2 Homo sapiens