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Literature summary extracted from
- Saturation mutagenesis of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase R308 site confirms its role in controlling substrate specificity (2011), Biotechnol. Lett., 33, 805-812.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.20.1 | R308A | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308C | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308D | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308E | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308F | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308G | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308H | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308I | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme. The mutant shows the highest reactivity for penicillin G, with 3fold increase in kcat/Km ratio and 7fold increase in relative activity | Acremonium chrysogenum |
| 1.14.20.1 | R308K | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308L | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308M | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308N | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308P | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308Q | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308S | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308T | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308V | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308W | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | R308Y | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.1 | 1.9 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum |  |
| 1.14.20.1 | 8.2 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
| 1.14.20.1 | 11 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.1 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetylcephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | additional information | Acremonium chrysogenum | a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C | ? | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.20.1 | Acremonium chrysogenum | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | additional information | - |
relative activities of wild-type and R308 mutants with penicillin variants and analogue substrates, overview | Acremonium chrysogenum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.1 | 6-aminopenicillanic acid + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | amoxicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | additional information | a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C | Acremonium chrysogenum | ? | - |
? | |
| 1.14.20.1 | additional information | role of residue R308 in controlling substrate selectivity, substrate specificity of wild-type and mutant enzymes, overview | Acremonium chrysogenum | ? | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin V + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.20.1 | acDAOC/DACS | - |
Acremonium chrysogenum |
| 1.14.20.1 | deacetoxy/deacetylcephalosporin C synthase | - |
Acremonium chrysogenum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.1 | 0.0057 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
| 1.14.20.1 | 0.048 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
| 1.14.20.1 | 0.069 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.20.1 | 0.003 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
| 1.14.20.1 | 0.0045 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
| 1.14.20.1 | 0.0085 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
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