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Literature summary extracted from

  • Qaed, A.A.; Lin, H.; Tang, D.F.; Wu, Z.L.
    Rational design of styrene monooxygenase mutants with altered substrate preference (2011), Biotechnol. Lett., 33, 611-616.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expression of mutant enzymes in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.11 L44A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida
1.14.14.11 L45A mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively Pseudomonas putida
1.14.14.11 N46A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida
1.14.14.11 R43A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida LQ26
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Pseudomonas putida O33471
-
-
1.14.14.11 Pseudomonas putida LQ26 O33471
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 2-ethylstyrene + FADH2 + O2 about 30% of the activity with styrene Pseudomonas putida (S)-2-ethyl-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 2-ethylstyrene + FADH2 + O2 about 30% of the activity with styrene Pseudomonas putida LQ26 (S)-2-ethyl-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 2-methylstyrene + FADH2 + O2 about 70% of the activity with styrene Pseudomonas putida (S)-2-methyl-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 2-methylstyrene + FADH2 + O2 about 70% of the activity with styrene Pseudomonas putida LQ26 (S)-2-methyl-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida LQ26 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 StyA
-
 Pseudomonas putida