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Literature summary extracted from

  • McClendon, S.D.; Shin, H.D.; Chen, R.R.
    Novel bacterial ferulic acid esterase from Cellvibrio japonicus and its application in ferulic acid release and xylan hydrolysis (2011), Biotechnol. Lett., 33, 47-54.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 genes fee1A and fee1B, DNA and mino acid sequence determination and analysis, subcloning in Escherichia coli strain JM109, expression as His-tagged Fee1B in Escherichia coli strain BL21(DE3) Cellvibrio japonicus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information generation of a truncated Feel1B variant with reduced enzyme activity Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Cellvibrio japonicus
-
 genes fee1A and fee1B
-
3.1.1.73 Cellvibrio japonicus NCIMB 10462
-
 genes fee1A and fee1B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant His-tagged Fee1B 12fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cellvibrio japonicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 5.4
-
 above, purified recombinant enzyme, substrate is methyl caffeate, pH 7.0, 40°C Cellvibrio japonicus
3.1.1.73 10.5
-
 above, purified recombinant enzyme, substrate is methyl sinapate, pH 7.0, 40°C Cellvibrio japonicus
3.1.1.73 12.4
-
 above, purified recombinant enzyme, substrate is methyl 4-coumarate, pH 7.0, 40°C Cellvibrio japonicus
3.1.1.73 28
-
 above, purified recombinant enzyme, substrate is methyl ferulate, pH 7.0, 40°C Cellvibrio japonicus
3.1.1.73 50
-
 purifed recombinant enzyme, substrate is 4-nitrophenyl acetate, pH 7.0, 40°C Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl acetate + H2O
-
 Cellvibrio japonicus 4-nitrophenol + acetate
-
 ?
3.1.1.73 4-nitrophenyl acetate + H2O
-
 Cellvibrio japonicus NCIMB 10462 4-nitrophenol + acetate
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Cellvibrio japonicus 4-coumaric acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Cellvibrio japonicus caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Cellvibrio japonicus NCIMB 10462 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Cellvibrio japonicus ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Cellvibrio japonicus NCIMB 10462 ferulic acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Cellvibrio japonicus sinapic acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Cellvibrio japonicus NCIMB 10462 sinapic acid + methanol
-
 ?
3.1.1.73 additional information substrate specificity of Fee1B, overview Cellvibrio japonicus ?
-
 ?
3.1.1.73 additional information substrate specificity of Fee1B, overview Cellvibrio japonicus NCIMB 10462 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Cellvibrio japonicus
3.1.1.73 Fee1B
-
 Cellvibrio japonicus
3.1.1.73 ferulic acid esterase
-
 Cellvibrio japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 35 40
-
 Cellvibrio japonicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 50 activity range of Fee1B, profile overview Cellvibrio japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 45
-
 purified recombinant Fee1B is stable at temperature up to 40°C. The enzyme loses about 30% activity upon incubation at 45°C for 30 min Cellvibrio japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.5
-
-
 Cellvibrio japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 4.5 10 activity range of Fee1B, profile overview Cellvibrio japonicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 additional information
-
 purified recombinant Fee1B is fairly stable over a wide range of pH Cellvibrio japonicus