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Literature summary extracted from

  • Zhang, L.Q.; Guo, F.X.; Xian, H.Q.; Wang, X.J.; Li, A.N.; Li, D.C.
    Expression of a novel thermostable Cu, Zn-superoxide dismutase from Chaetomium thermophilum in Pichia pastoris and its antioxidant properties (2011), Biotechnol. Lett., 33, 1127-1132.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.15.1.1 biotechnology transfection of the Ctsod gene may have great potential for the genetic improvement of salt tolerance in plants Thermochaetoides thermophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene Ctsod, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strains DH5a and JM109, expression in Pichia pastoris strain GS115, the recombinant yeast exhibit higher stress resistance than the control yeast cells to salt and superoxide-generating agents, such as paraquat and menadione Thermochaetoides thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2
-
 Thermochaetoides thermophila
1.15.1.1 KCN
-
 Thermochaetoides thermophila
1.15.1.1 additional information no inhibition by NaN3 Thermochaetoides thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a CuZn-superoxide dismutase Thermochaetoides thermophila
1.15.1.1 Zn2+ a CuZn-superoxide dismutase Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Thermochaetoides thermophila Q1HEQ0 gene Ctsod
-
1.15.1.1 Thermochaetoides thermophila HSAUP072651 Q1HEQ0 gene Ctsod
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant Cu, ZnSOD from Pichia pastoris strain GS115 by anion exchange chromatography Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 2170
-
 purified recombinant enzyme, pH 6.5, 60°C Thermochaetoides thermophila

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 CtSOD
-
 Thermochaetoides thermophila
1.15.1.1 Cu, Zn-superoxide dismutase
-
 Thermochaetoides thermophila
1.15.1.1 Cu, ZnSOD
-
 Thermochaetoides thermophila
1.15.1.1 SOD
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 60
-
 recombinant enzyme Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 70
-
 purified recombinant enzyme, retains 65% of the maximum activity at 70°C for 60 min Thermochaetoides thermophila
1.15.1.1 80
-
 purified recombinant enzyme, half-life is 22 min Thermochaetoides thermophila
1.15.1.1 90
-
 purified recombinant enzyme, half-life is 7 min Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 6.5
-
 recombinant enzyme Thermochaetoides thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 5 10 activity profile, overview Thermochaetoides thermophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 5 9 the purified recombinant enzyme is relatively stable at pH 5.0-9.0, and retains more than 70% of full activity at pH 5.5-8.0 Thermochaetoides thermophila